{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kundu S"],"funding":["National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["9576-9584"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11299218"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["96(23)"],"pubmed_abstract":["GPI-anchored proteins (GPI-APs) are ubiquitous and essential but exist in low abundances on the cell surface, making their analysis and investigation especially challenging. To tackle the problem, a new method to detect and study GPI-APs based upon GPI metabolic engineering and DNA-facilitated fluorescence signal amplification was developed. In this context, cell surface GPI-APs were metabolically engineered using azido-inositol derivatives to introduce an azido group. This allowed GPI-AP coupling with alkyne-functionalized multifluorophore DNA assemblies generated by hybridization chain reaction (HCR). It was demonstrated that this approach could significantly improve the detection limit and sensitivity of GPI-APs, thereby enabling various biological studies, including the investigation o"],"journal":["Analytical chemistry"],"pubmed_title":["Sensitive Method To Analyze Cell Surface GPI-Anchored Proteins Using DNA Hybridization Chain Reaction-Mediated Signal Amplification."],"pmcid":["PMC11299218"],"funding_grant_id":["R35 GM131686","GM131686"],"pubmed_authors":["Guo J","Guo Z","Craig KC","Gupta P","Jaiswal M","Kundu S"],"additional_accession":[]},"is_claimable":false,"name":"Sensitive Method To Analyze Cell Surface GPI-Anchored Proteins Using DNA Hybridization Chain Reaction-Mediated Signal Amplification.","description":"GPI-anchored proteins (GPI-APs) are ubiquitous and essential but exist in low abundances on the cell surface, making their analysis and investigation especially challenging. To tackle the problem, a new method to detect and study GPI-APs based upon GPI metabolic engineering and DNA-facilitated fluorescence signal amplification was developed. In this context, cell surface GPI-APs were metabolically engineered using azido-inositol derivatives to introduce an azido group. This allowed GPI-AP coupling with alkyne-functionalized multifluorophore DNA assemblies generated by hybridization chain reaction (HCR). It was demonstrated that this approach could significantly improve the detection limit and sensitivity of GPI-APs, thereby enabling various biological studies, including the investigation o","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2025-04-27T00:14:46.223Z","creation":"2025-04-06T17:47:18.112Z"},"accession":"S-EPMC11299218","cross_references":{"pubmed":["38808923"],"doi":["10.1021/acs.analchem.4c01116"]}}