biostudies-literatureGu YNational Natural Science Foundation of China2378210https://www.ebi.ac.uk/biostudies/studies/S-EPMC11299449biostudies-literatureUnknown46(2)<b>Objectives:</b> To explore the therapeutic effects of M2 macrophages in diabetic nephropathy (DN) and their mechanism.<b>Methods:</b> We infused M2 macrophages stimulated with IL-4 into 10-week-old db/db mice once a week for 4 weeks through the tail vein as M2 group. Then we investigated the role of M2 macrophages in alleviating the infammation of DN and explored the mechanism.<b>Results:</b> M2 macrophages hindered the progression of DN, reduced the levels of IL-1β (DN group was 34%, M2 group was 13%, <i>p</i> < 0.01) and MCP-1 (DN group was 49%, M2 group was 16%, <i>p</i> < 0.01) in the glomeruli. It was also proven that M2 macrophages alleviate mesangial cell injury caused by a high glucose environment. M2 macrophage tracking showed that the infused M2 macrophages migrated to the kidRenal failureM2 macrophage infusion ameliorates diabetic glomerulopathy <i>via</i> the JAK2/STAT3 pathway in db/db mice.PMC1129944981870578Yin YLiu JGu YYu SLyu ZMu YGu WZhang HYuan MGuo QZhang QCheng YLi BXue JfalseM2 macrophage infusion ameliorates diabetic glomerulopathy <i>via</i> the JAK2/STAT3 pathway in db/db mice.<b>Objectives:</b> To explore the therapeutic effects of M2 macrophages in diabetic nephropathy (DN) and their mechanism.<b>Methods:</b> We infused M2 macrophages stimulated with IL-4 into 10-week-old db/db mice once a week for 4 weeks through the tail vein as M2 group. Then we investigated the role of M2 macrophages in alleviating the infammation of DN and explored the mechanism.<b>Results:</b> M2 macrophages hindered the progression of DN, reduced the levels of IL-1β (DN group was 34%, M2 group was 13%, <i>p</i> < 0.01) and MCP-1 (DN group was 49%, M2 group was 16%, <i>p</i> < 0.01) in the glomeruli. It was also proven that M2 macrophages alleviate mesangial cell injury caused by a high glucose environment. M2 macrophage tracking showed that the infused M2 macrophages migrated to the kid2024-01-01T00:00:00Z2024 Dec2025-04-05T15:02:52.712Z2025-02-19T04:41:01.013ZS-EPMC112994493909096610.1080/0886022X.2024.2378210