biostudies-literatureZhu DNICHD NIH HHSNHLBI NIH HHSNCI NIH HHSNLM NIH HHS107494https://www.ebi.ac.uk/biostudies/studies/S-EPMC11301355biostudies-literatureUnknown300(8)The commitment of stem cells to differentiate into osteoblasts is a highly regulated and complex process that involves the coordination of extrinsic signals and intrinsic transcriptional machinery. While rodent osteoblastic differentiation has been extensively studied, research on human osteogenesis has been limited by cell sources and existing models. Here, we systematically dissect human pluripotent stem cell-derived osteoblasts to identify functional membrane proteins and their downstream transcriptional networks involved in human osteogenesis. Our results reveal an enrichment of type II transmembrane serine protease CORIN in humans but not rodent osteoblasts. Functional analyses demonstrated that CORIN depletion significantly impairs osteogenesis. Genome-wide chromatin immunoprecipitatThe Journal of biological chemistrySystematic transcriptome profiling of hPSC-derived osteoblasts unveils CORIN's mastery in governing osteogenesis through CEBPD modulation.PMC11301355R01 CA246130R01 HL142704K08 HD042136R01 LM012806Huang YWPang LKShen JBazer DATu JKameoka JWang JZhao ZZhu DDai YAmbrose CGLu LHuo ZPhan TTTShoemaker RZhao RZhang YXu AGingold JAGao XLee DFWang LLHuang MFChi TYfalseSystematic transcriptome profiling of hPSC-derived osteoblasts unveils CORIN's mastery in governing osteogenesis through CEBPD modulation.The commitment of stem cells to differentiate into osteoblasts is a highly regulated and complex process that involves the coordination of extrinsic signals and intrinsic transcriptional machinery. While rodent osteoblastic differentiation has been extensively studied, research on human osteogenesis has been limited by cell sources and existing models. Here, we systematically dissect human pluripotent stem cell-derived osteoblasts to identify functional membrane proteins and their downstream transcriptional networks involved in human osteogenesis. Our results reveal an enrichment of type II transmembrane serine protease CORIN in humans but not rodent osteoblasts. Functional analyses demonstrated that CORIN depletion significantly impairs osteogenesis. Genome-wide chromatin immunoprecipitat2024-01-01T00:00:00Z2024 Aug2026-06-02T22:54:39.325Z2024-11-09T02:03:44.016ZS-EPMC113013553892532610.1016/j.jbc.2024.107494