{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sarkar K"],"funding":["NIDCR NIH HHS","NIA NIH HHS","National Institutes of Health","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["2580-2596.e6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11301765"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["58(22)"],"pubmed_abstract":["Germ cells differentiate into oocytes that launch the next generation upon fertilization. How the highly specialized oocyte acquires this distinct cell fate is poorly understood. During Drosophila oogenesis, H3K9me3 histone methyltransferase SETDB1 translocates from the cytoplasm to the nucleus of germ cells concurrently with oocyte specification. Here, we discovered that nuclear SETDB1 is required for silencing a cohort of differentiation-promoting genes by mediating their heterochromatinization. Intriguingly, SETDB1 is also required for upregulating 18 of the ∼30 nucleoporins (Nups) that compose the nucleopore complex (NPC), promoting NPC formation. NPCs anchor SETDB1-dependent heterochromatin at the nuclear periphery to maintain H3K9me3 and gene silencing in the egg chambers. Aberrant g"],"journal":["Developmental cell"],"pubmed_title":["A feedback loop between heterochromatin and the nucleopore complex controls germ-cell-to-oocyte transition during Drosophila oogenesis."],"pmcid":["PMC11301765"],"funding_grant_id":["RO1GM11177","R01 GM135628","R35 138120","R01 GM111779","R35 GM138120","R01 GM111772","R01 GM084947","R01DE030927","RO1GM135628","R56 AG082906","R01 DE030927"],"pubmed_authors":["Kotb NM","Valm AM","Lemus A","Sarkar K","Iqbal A","McCarthy A","Martin ET","Camacho J","Rangan P","Sammons MA"],"additional_accession":[]},"is_claimable":false,"name":"A feedback loop between heterochromatin and the nucleopore complex controls germ-cell-to-oocyte transition during Drosophila oogenesis.","description":"Germ cells differentiate into oocytes that launch the next generation upon fertilization. How the highly specialized oocyte acquires this distinct cell fate is poorly understood. During Drosophila oogenesis, H3K9me3 histone methyltransferase SETDB1 translocates from the cytoplasm to the nucleus of germ cells concurrently with oocyte specification. Here, we discovered that nuclear SETDB1 is required for silencing a cohort of differentiation-promoting genes by mediating their heterochromatinization. Intriguingly, SETDB1 is also required for upregulating 18 of the ∼30 nucleoporins (Nups) that compose the nucleopore complex (NPC), promoting NPC formation. NPCs anchor SETDB1-dependent heterochromatin at the nuclear periphery to maintain H3K9me3 and gene silencing in the egg chambers. Aberrant g","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-06-01T15:22:41.202Z","creation":"2025-04-04T02:33:57.664Z"},"accession":"S-EPMC11301765","cross_references":{"pubmed":["37673064"],"doi":["10.1016/j.devcel.2023.08.014"]}}