biostudies-literatureUnknown24(1)Chow JYLee Kong Chian School of Medicine—Ministry of Education Start-Up Grant<h4>Background</h4>Wolbachia symbiosis in Aedes aegypti is an emerging biocontrol measure against dengue. However, assessing its real-world efficacy is challenging due to the non-randomised, field-based nature of most intervention studies. This research re-evaluates the spatial-temporal impact of Wolbachia interventions on dengue incidence using a large battery of quasi-experimental methods and assesses each method's validity.<h4>Methods</h4>A systematic search for Wolbachia intervention data was conducted via PUBMED. Efficacy was reassessed using commonly-used quasi-experimental approaches with extensive robustness checks, including geospatial placebo tests and a simulation study. Intervention efficacies across multiple study sites were computed using high-resolution aggregations to examiBMC medical research methodology170https://www.ebi.ac.uk/biostudies/studies/S-EPMC11302844biostudies-literatureEvaluating quasi-experimental approaches for estimating epidemiological efficacy of non-randomised field trials: applications in Wolbachia interventions for dengue.PMC11302844Lim JTHoffmann AABansal SNg LCGeng LDickens BSLChow JYfalseEvaluating quasi-experimental approaches for estimating epidemiological efficacy of non-randomised field trials: applications in Wolbachia interventions for dengue.<h4>Background</h4>Wolbachia symbiosis in Aedes aegypti is an emerging biocontrol measure against dengue. However, assessing its real-world efficacy is challenging due to the non-randomised, field-based nature of most intervention studies. This research re-evaluates the spatial-temporal impact of Wolbachia interventions on dengue incidence using a large battery of quasi-experimental methods and assesses each method's validity.<h4>Methods</h4>A systematic search for Wolbachia intervention data was conducted via PUBMED. Efficacy was reassessed using commonly-used quasi-experimental approaches with extensive robustness checks, including geospatial placebo tests and a simulation study. Intervention efficacies across multiple study sites were computed using high-resolution aggregations to exami2024-01-01T00:00:00Z2024 Aug2025-04-04T02:57:31.696Z2025-02-19T04:40:01.802ZS-EPMC113028443910771010.1186/s12874-024-02291-6