{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["170(8)"],"submitter":["Alfahl Z"],"funding":["Environmental Protection Agency"],"pubmed_abstract":["Shiga toxin-producing <i>Escherichia coli</i> (STEC) is an important waterborne pathogen capable of causing serious gastrointestinal infections with potentially fatal complications, including haemolytic-uremic syndrome. All STEC serogroups harbour genes that encode at least one Shiga toxin (<i>stx1</i> and/or <i>stx2</i>), which constitute the primary virulence factors of STEC. Loop-mediated isothermal amplification (LAMP) enables rapid real-time pathogen detection with a high degree of specificity and sensitivity. The aim of this study was to develop and validate an on-site portable diagnostics workstation employing LAMP technology to permit rapid real-time STEC detection in environmental water samples. Water samples (<i>n</i>=28) were collected from groundwater wells (<i>n</i>=13), river"],"journal":["Microbiology (Reading, England)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11304963"],"repository":["biostudies-literature"],"pubmed_title":["A rapid on-site loop-mediated isothermal amplification technology as an early warning system for the detection of Shiga toxin-producing <i>Escherichia coli</i> in water."],"pmcid":["PMC11304963"],"funding_grant_id":["2021-HE-1033"],"pubmed_authors":["O'Connor L","Higgins O","Alfahl Z","O'Dwyer J","Hynds PD","Biggins S","Burke LP","Smith TJ","Chueiri A","Morris D"],"additional_accession":[]},"is_claimable":false,"name":"A rapid on-site loop-mediated isothermal amplification technology as an early warning system for the detection of Shiga toxin-producing <i>Escherichia coli</i> in water.","description":"Shiga toxin-producing <i>Escherichia coli</i> (STEC) is an important waterborne pathogen capable of causing serious gastrointestinal infections with potentially fatal complications, including haemolytic-uremic syndrome. All STEC serogroups harbour genes that encode at least one Shiga toxin (<i>stx1</i> and/or <i>stx2</i>), which constitute the primary virulence factors of STEC. Loop-mediated isothermal amplification (LAMP) enables rapid real-time pathogen detection with a high degree of specificity and sensitivity. The aim of this study was to develop and validate an on-site portable diagnostics workstation employing LAMP technology to permit rapid real-time STEC detection in environmental water samples. Water samples (<i>n</i>=28) were collected from groundwater wells (<i>n</i>=13), river","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2025-04-05T11:09:27.396Z","creation":"2025-04-05T11:09:27.396Z"},"accession":"S-EPMC11304963","cross_references":{"pubmed":["39109421"],"doi":["10.1099/mic.0.001485"]}}