biostudies-literatureUnknown170(8)Alfahl ZEnvironmental Protection AgencyShiga toxin-producing <i>Escherichia coli</i> (STEC) is an important waterborne pathogen capable of causing serious gastrointestinal infections with potentially fatal complications, including haemolytic-uremic syndrome. All STEC serogroups harbour genes that encode at least one Shiga toxin (<i>stx1</i> and/or <i>stx2</i>), which constitute the primary virulence factors of STEC. Loop-mediated isothermal amplification (LAMP) enables rapid real-time pathogen detection with a high degree of specificity and sensitivity. The aim of this study was to develop and validate an on-site portable diagnostics workstation employing LAMP technology to permit rapid real-time STEC detection in environmental water samples. Water samples (<i>n</i>=28) were collected from groundwater wells (<i>n</i>=13), riverMicrobiology (Reading, England)https://www.ebi.ac.uk/biostudies/studies/S-EPMC11304963biostudies-literatureA rapid on-site loop-mediated isothermal amplification technology as an early warning system for the detection of Shiga toxin-producing <i>Escherichia coli</i> in water.PMC113049632021-HE-1033O'Connor LHiggins OAlfahl ZO'Dwyer JHynds PDBiggins SBurke LPSmith TJChueiri AMorris DfalseA rapid on-site loop-mediated isothermal amplification technology as an early warning system for the detection of Shiga toxin-producing <i>Escherichia coli</i> in water.Shiga toxin-producing <i>Escherichia coli</i> (STEC) is an important waterborne pathogen capable of causing serious gastrointestinal infections with potentially fatal complications, including haemolytic-uremic syndrome. All STEC serogroups harbour genes that encode at least one Shiga toxin (<i>stx1</i> and/or <i>stx2</i>), which constitute the primary virulence factors of STEC. Loop-mediated isothermal amplification (LAMP) enables rapid real-time pathogen detection with a high degree of specificity and sensitivity. The aim of this study was to develop and validate an on-site portable diagnostics workstation employing LAMP technology to permit rapid real-time STEC detection in environmental water samples. Water samples (<i>n</i>=28) were collected from groundwater wells (<i>n</i>=13), river2024-01-01T00:00:00Z2024 Aug2025-04-05T11:09:27.396Z2025-04-05T11:09:27.396ZS-EPMC113049633910942110.1099/mic.0.001485