{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tao CY"],"funding":["Shanghai Municipal Science and Technology Major Project","Shanghai Municipal Health Commission","National Key R&amp;D Program of China","National Key R&D Program of China","Shanghai Municipal Key Clinical Specialty. CAMS Innovation Fund for Medical Sciences (CIFMS)","National Natural Science Foundation of China","Clinical Research Plan of SHDC","Shanghai Municipal Key Clinical Specialty. CAMS Innovation Fund for Medical Sciences"],"pagination":["1391-1403"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11322209"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["47(4)"],"pubmed_abstract":["<h4>Purpose</h4>GPX8, which is found in the endoplasmic reticulum lumen, is a member of the Glutathione Peroxidases (GPXs) family. Its role in hepatocellular carcinoma (HCC) is unknown.<h4>Methods</h4>Immunohistochemical staining was used to detect the protein levels of GPX8 in HCC tissue microarrays. A short hairpin RNA lentivirus was used to knock down GPX8, and the main signaling pathways were investigated using transcriptome sequencing and a phosphorylated kinase array. The sphere formation assays, cloning-formation assays and cell migration assays were used to evaluate the stemness and migration ability of HCC cells. Identifying the GPX8-interacting proteins was accomplished through immunoprecipitation and protein mass spectrometry.<h4>Results</h4>The GPX8 protein levels were downregu"],"journal":["Cellular oncology (Dordrecht, Netherlands)"],"pubmed_title":["Downregulation of GPX8 in hepatocellular carcinoma: impact on tumor stemness and migration."],"pmcid":["PMC11322209"],"funding_grant_id":["202040221","82073217","2018YFC1312100","No. SHDC2020CR5007","2019-I2M-5-058","2018SHZDZX05"],"pubmed_authors":["Zhu GQ","Qu WF","Tang Z","Zhou YF","Song SS","Chu TH","Tian MX","Wu XL","Gao J","Zhao QF","Huang R","Dai Z","Liu WR","Fang Y","Tao CY","Chen JF","Fan J","Shi YH","Ding ZB","Yang R","Jiang XF","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"Downregulation of GPX8 in hepatocellular carcinoma: impact on tumor stemness and migration.","description":"<h4>Purpose</h4>GPX8, which is found in the endoplasmic reticulum lumen, is a member of the Glutathione Peroxidases (GPXs) family. Its role in hepatocellular carcinoma (HCC) is unknown.<h4>Methods</h4>Immunohistochemical staining was used to detect the protein levels of GPX8 in HCC tissue microarrays. A short hairpin RNA lentivirus was used to knock down GPX8, and the main signaling pathways were investigated using transcriptome sequencing and a phosphorylated kinase array. The sphere formation assays, cloning-formation assays and cell migration assays were used to evaluate the stemness and migration ability of HCC cells. Identifying the GPX8-interacting proteins was accomplished through immunoprecipitation and protein mass spectrometry.<h4>Results</h4>The GPX8 protein levels were downregu","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2025-05-18T12:13:57.433Z","creation":"2025-04-19T13:51:18.719Z"},"accession":"S-EPMC11322209","cross_references":{"pubmed":["38607517"],"doi":["10.1007/s13402-024-00934-w"]}}