biostudies-literatureTao CYShanghai Municipal Science and Technology Major ProjectShanghai Municipal Health CommissionNational Key R&amp;D Program of ChinaNational Key R&D Program of ChinaShanghai Municipal Key Clinical Specialty. CAMS Innovation Fund for Medical Sciences (CIFMS)National Natural Science Foundation of ChinaClinical Research Plan of SHDCShanghai Municipal Key Clinical Specialty. CAMS Innovation Fund for Medical Sciences1391-1403https://www.ebi.ac.uk/biostudies/studies/S-EPMC11322209biostudies-literatureUnknown47(4)<h4>Purpose</h4>GPX8, which is found in the endoplasmic reticulum lumen, is a member of the Glutathione Peroxidases (GPXs) family. Its role in hepatocellular carcinoma (HCC) is unknown.<h4>Methods</h4>Immunohistochemical staining was used to detect the protein levels of GPX8 in HCC tissue microarrays. A short hairpin RNA lentivirus was used to knock down GPX8, and the main signaling pathways were investigated using transcriptome sequencing and a phosphorylated kinase array. The sphere formation assays, cloning-formation assays and cell migration assays were used to evaluate the stemness and migration ability of HCC cells. Identifying the GPX8-interacting proteins was accomplished through immunoprecipitation and protein mass spectrometry.<h4>Results</h4>The GPX8 protein levels were downreguCellular oncology (Dordrecht, Netherlands)Downregulation of GPX8 in hepatocellular carcinoma: impact on tumor stemness and migration.PMC11322209202040221820732172018YFC1312100No. SHDC2020CR50072019-I2M-5-0582018SHZDZX05Zhu GQQu WFTang ZZhou YFSong SSChu THTian MXWu XLGao JZhao QFHuang RDai ZLiu WRFang YTao CYChen JFFan JShi YHDing ZBYang RJiang XFZhou JfalseDownregulation of GPX8 in hepatocellular carcinoma: impact on tumor stemness and migration.<h4>Purpose</h4>GPX8, which is found in the endoplasmic reticulum lumen, is a member of the Glutathione Peroxidases (GPXs) family. Its role in hepatocellular carcinoma (HCC) is unknown.<h4>Methods</h4>Immunohistochemical staining was used to detect the protein levels of GPX8 in HCC tissue microarrays. A short hairpin RNA lentivirus was used to knock down GPX8, and the main signaling pathways were investigated using transcriptome sequencing and a phosphorylated kinase array. The sphere formation assays, cloning-formation assays and cell migration assays were used to evaluate the stemness and migration ability of HCC cells. Identifying the GPX8-interacting proteins was accomplished through immunoprecipitation and protein mass spectrometry.<h4>Results</h4>The GPX8 protein levels were downregu2024-01-01T00:00:00Z2024 Aug2025-05-18T12:13:57.433Z2025-04-19T13:51:18.719ZS-EPMC113222093860751710.1007/s13402-024-00934-w