{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bouin A"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH)","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","U.S. Department of Health & Human Services | U.S. Food and Drug Administration","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)","NIDA NIH HHS","NIAID NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","NIMH NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS)","U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)","U.S. Department of Health & Human Services | NIH | NIH Office of the Director","NSF | BIO | Division of Biological Infrastructure","U.S. Department of Health & Human Services | NIH | National Institute of Mental Health","U.S. Department of Health & Human Services | NIH | NIH Office of the Director (OD)","U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke","NSF | BIO | Division of Biological Infrastructure (DBI)","FDA HHS","NINDS NIH HHS","U.S. Department of Health & Human Services | U.S. Food and Drug Administration (U.S. Food & Drug Administration)","NIH HHS","NIGMS NIH HHS"],"pagination":["1951-1967"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11322437"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["29(7)"],"pubmed_abstract":["Comparisons and linkage between multiple imaging scales are essential for neural circuit connectomics. Here, we report 20 new recombinant rabies virus (RV) vectors that we have developed for multi-scale and multi-modal neural circuit mapping tools. Our new RV tools for mesoscale imaging express a range of improved fluorescent proteins. Further refinements target specific neuronal subcellular locations of interest. We demonstrate the discovery power of these new tools including the detection of detailed microstructural changes of rabies-labeled neurons in aging and Alzheimer's disease mouse models, live imaging of neuronal activities using calcium indicators, and automated measurement of infected neurons. RVs that encode GFP and ferritin as electron microscopy (EM) and fluorescence microsco"],"journal":["Molecular psychiatry"],"pubmed_title":["New rabies viral resources for multi-scale neural circuit mapping."],"pmcid":["PMC11322437"],"funding_grant_id":["RF1 MH120020","5R35GM127102","R21 AI144492","NSF2014862-UTA20-000890","1U24NS120055","RF1MH120020","R35 GM127102","R24 GM137200","R01 FD007478","R21AI144492","R24GM137200","R01FD007478","S10 OD021784","R01 DA038896","S10 MH124715","1S10OD021784","5R01GM082949","U24 NS120055"],"pubmed_authors":["Ellisman MH","Ramachandra R","Tong L","Chen L","Bouin A","Kim KY","Wu G","Semler BL","Wu MY","Phan S","Ye Q","Koyuncu OO","Mackey MR","Xu X","Holmes TC"],"additional_accession":[]},"is_claimable":false,"name":"New rabies viral resources for multi-scale neural circuit mapping.","description":"Comparisons and linkage between multiple imaging scales are essential for neural circuit connectomics. Here, we report 20 new recombinant rabies virus (RV) vectors that we have developed for multi-scale and multi-modal neural circuit mapping tools. Our new RV tools for mesoscale imaging express a range of improved fluorescent proteins. Further refinements target specific neuronal subcellular locations of interest. We demonstrate the discovery power of these new tools including the detection of detailed microstructural changes of rabies-labeled neurons in aging and Alzheimer's disease mouse models, live imaging of neuronal activities using calcium indicators, and automated measurement of infected neurons. RVs that encode GFP and ferritin as electron microscopy (EM) and fluorescence microsco","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-04-21T22:19:56.013Z","creation":"2025-04-05T18:43:00.495Z"},"accession":"S-EPMC11322437","cross_references":{"pubmed":["38355784"],"doi":["10.1038/s41380-024-02451-6"]}}