biostudies-literatureBatliner MDeutsche Forschungsgemeinschaft (DFG)Deutsche Forschungsgemeinschafte0073224https://www.ebi.ac.uk/biostudies/studies/S-EPMC11323541biostudies-literatureUnknown15(8)<i>Candida albicans,</i> an opportunistic fungal pathogen, produces the quorum-sensing molecule farnesol, which we have shown alters the transcriptional response and phenotype of human monocyte-derived dendritic cells (DCs), including their cytokine secretion and ability to prime T cells. This is partially dependent on the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ), which has numerous ligands, including the sphingolipid metabolite sphingosine 1-phosphate. Sphingolipids are a vital component of membranes that affect membrane protein arrangement and phagocytosis of <i>C. albicans</i> by DCs. Thus, we quantified sphingolipid metabolites in monocytes differentiating into DCs by High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). FarnesomBioThe <i>Candida albicans</i> quorum-sensing molecule farnesol alters sphingolipid metabolism in human monocyte-derived dendritic cells.PMC11323541417857878492620490210879364Riedel ABatliner MSchempp RFekete AKohler TWigger DSchumacher FFohmann IKurzai OPrell AVaeth MKleuser BVivas WNieuwenhuizen NEfalseThe <i>Candida albicans</i> quorum-sensing molecule farnesol alters sphingolipid metabolism in human monocyte-derived dendritic cells.<i>Candida albicans,</i> an opportunistic fungal pathogen, produces the quorum-sensing molecule farnesol, which we have shown alters the transcriptional response and phenotype of human monocyte-derived dendritic cells (DCs), including their cytokine secretion and ability to prime T cells. This is partially dependent on the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ), which has numerous ligands, including the sphingolipid metabolite sphingosine 1-phosphate. Sphingolipids are a vital component of membranes that affect membrane protein arrangement and phagocytosis of <i>C. albicans</i> by DCs. Thus, we quantified sphingolipid metabolites in monocytes differentiating into DCs by High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Farneso2024-01-01T00:00:00Z2024 Aug2025-04-19T20:39:22.825Z2025-04-19T20:39:22.825ZS-EPMC113235413895335310.1128/mbio.00732-24