biostudies-literatureParthasarathy ANIGMS NIH HHS1456027https://www.ebi.ac.uk/biostudies/studies/S-EPMC11324575biostudies-literatureUnknown15Antimicrobial resistance (AMR) is one of the most alarming global public health challenges of the 21st century. Over 3 million antimicrobial-resistant infections occur in the United States annually, with nearly 50,000 cases being fatal. Innovations in drug discovery methods and platforms are crucial to identify novel antibiotics to combat AMR. We present the isolation and characterization of potentially novel antibiotic lead compounds produced by the cross-feeding of two rhizosphere bacteria, <i>Acinetobacter</i> sp. RIT 592 and <i>Exiguobacterium</i> sp. RIT 594. We used solid-phase extraction (SPE) followed by liquid chromatography (LC) to enrich antibiotic extracts and subsequently mass spectrometry (MS) analysis of collected fractions for compound structure identification and characterFrontiers in pharmacologyInteraction of <i>Acinetobacter</i> sp. RIT 592 induces the production of broad-spectrum antibiotics in <i>Exiguobacterium</i> sp. RIT 594.PMC11324575R15 GM144862Parthasarathy AMantravadi PKWong NHMiranda RRBulman ZPAdjei JAHudson AORana APWatts LMBorrego EJChu JBedore TJSavka MAfalseInteraction of <i>Acinetobacter</i> sp. RIT 592 induces the production of broad-spectrum antibiotics in <i>Exiguobacterium</i> sp. RIT 594.Antimicrobial resistance (AMR) is one of the most alarming global public health challenges of the 21st century. Over 3 million antimicrobial-resistant infections occur in the United States annually, with nearly 50,000 cases being fatal. Innovations in drug discovery methods and platforms are crucial to identify novel antibiotics to combat AMR. We present the isolation and characterization of potentially novel antibiotic lead compounds produced by the cross-feeding of two rhizosphere bacteria, <i>Acinetobacter</i> sp. RIT 592 and <i>Exiguobacterium</i> sp. RIT 594. We used solid-phase extraction (SPE) followed by liquid chromatography (LC) to enrich antibiotic extracts and subsequently mass spectrometry (MS) analysis of collected fractions for compound structure identification and character2024-01-01T00:00:00Z20242025-04-04T00:08:56.171Z2025-04-04T00:08:56.171ZS-EPMC113245753914855110.3389/fphar.2024.1456027