biostudies-literatureMarquez MCCR NIH HHSNational Institute of Allergy and Infectious DiseasesNational Cancer InstituteNCI NIH HHSNational Institutes of Health106990https://www.ebi.ac.uk/biostudies/studies/S-EPMC11330712biostudies-literatureUnknown224The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p < 0.001) than other PP. The use of the conical-PP can save research budgets in the current inflationary environment.Journal of microbiological methodsEscherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector.PMC11330712HHSN261200800001EHHSN261200800001CImamichi TChen QYang JMarquez MSui HCachaco SfalseEscherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector.The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p < 0.001) than other PP. The use of the conical-PP can save research budgets in the current inflationary environment.2024-01-01T00:00:00Z2024 Sep2026-04-08T13:39:34.351Z2026-04-08T03:15:47.55ZS-EPMC113307123900428510.1016/j.mimet.2024.106990