biostudies-literatureUnknown11Yaneselli KPlatelet lysate (PL) is investigated as a potential replacement for fetal bovine serum (FBS) in cell culture. However, there is limited research on its impact on the immune profile of equine mesenchymal stromal cells (eMSCs). This study aimed to evaluate the effects of different PL formulations on the proliferative capacity, multipotentiality, and immune profile of equine adipose tissue-derived MSCs (eAD-MSCs). <i>In vitro</i> growth kinetics and trilineage differentiation of eAD-MSCs (<i>n</i> = 7) were assessed under three culture conditions: medium-concentration PL (MPL), high-concentration PL (HPL), and FBS as a control. The immune profile was evaluated by studying the expression of immunogenic receptors such as MHC I, MHC II, and immunomodulatory molecules IL-6, IL-10, and TNF-α, deteFrontiers in veterinary science1410855https://www.ebi.ac.uk/biostudies/studies/S-EPMC11330840biostudies-literatureImpact of different formulations of platelet lysate on proliferative and immune profile of equine mesenchymal stromal cells.PMC11330840Rial ASuarez GEstrade MJAlgorta AAvila GYaneselli KRossi ACastro SfalseImpact of different formulations of platelet lysate on proliferative and immune profile of equine mesenchymal stromal cells.Platelet lysate (PL) is investigated as a potential replacement for fetal bovine serum (FBS) in cell culture. However, there is limited research on its impact on the immune profile of equine mesenchymal stromal cells (eMSCs). This study aimed to evaluate the effects of different PL formulations on the proliferative capacity, multipotentiality, and immune profile of equine adipose tissue-derived MSCs (eAD-MSCs). <i>In vitro</i> growth kinetics and trilineage differentiation of eAD-MSCs (<i>n</i> = 7) were assessed under three culture conditions: medium-concentration PL (MPL), high-concentration PL (HPL), and FBS as a control. The immune profile was evaluated by studying the expression of immunogenic receptors such as MHC I, MHC II, and immunomodulatory molecules IL-6, IL-10, and TNF-α, dete2024-01-01T00:00:00Z20242026-04-22T03:22:07.004Z2025-05-18T12:15:20.617ZS-EPMC113308403916146010.3389/fvets.2024.1410855