biostudies-literatureYang LNational Natural Science Foundation of China for Young Scholars111https://www.ebi.ac.uk/biostudies/studies/S-EPMC11334498biostudies-literatureUnknown29(1)<h4>Objective</h4>Colorectal cancer (CRC) is a form of malignancy that exhibits a comparatively elevated occurrence and fatality rate. Given the relatively slower progress in diagnostic and therapeutic approaches for CRC, there is a need to investigate more accurate and efficient biomarkers.<h4>Methods</h4>Core regulatory genes were screened using the TCGA database, and the expression of neurexophilin 4 (NXPH4) and its prognostic implications were validated using tissue microarray staining. The assessment of NXPH4 functions involved a range of experiments, including cellular, organoid, and murine models. Furthermore, a regulatory network between m⁵C, NXPH4, and HIF1A was established through several in vitro experiments.<h4>Results</h4>The overexpression of NXPH4 is associated wiCellular & molecular biology lettersNXPH4 mediated by m⁵C contributes to the malignant characteristics of colorectal cancer via inhibiting HIF1A degradation.PMC1133449882303877Xu LLian ZYang LHuang JHe GXu HWu HMiao KYin HSun PZhang XShi JWang ZZhong MfalseNXPH4 mediated by m⁵C contributes to the malignant characteristics of colorectal cancer via inhibiting HIF1A degradation.<h4>Objective</h4>Colorectal cancer (CRC) is a form of malignancy that exhibits a comparatively elevated occurrence and fatality rate. Given the relatively slower progress in diagnostic and therapeutic approaches for CRC, there is a need to investigate more accurate and efficient biomarkers.<h4>Methods</h4>Core regulatory genes were screened using the TCGA database, and the expression of neurexophilin 4 (NXPH4) and its prognostic implications were validated using tissue microarray staining. The assessment of NXPH4 functions involved a range of experiments, including cellular, organoid, and murine models. Furthermore, a regulatory network between m⁵C, NXPH4, and HIF1A was established through several in vitro experiments.<h4>Results</h4>The overexpression of NXPH4 is associated wi2024-01-01T00:00:00Z2024 Aug2026-06-03T01:00:17.105Z2024-10-18T19:42:10.299ZS-EPMC113344983916464110.1186/s11658-024-00630-5