{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ding Y"],"funding":["the National Key Research and Development Progragm of China","Natural Science Foundation of Chongqing","the Fundamental Research Funds for the Central Universities","National Natural Science Foundation of China"],"pagination":["494"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11334516"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(1)"],"pubmed_abstract":["<h4>Background</h4>Sclerotinia sclerotiorum is a highly destructive phytopathogenic fungus that poses a significant threat to a wide array of crops. The current constraints in genetic manipulation techniques impede a thorough comprehension of its pathogenic mechanisms and the development of effective control strategies.<h4>Results</h4>Herein, we present a highly efficient genetic transformation system for S. sclerotiorum, leveraging the use of fusiform nanoparticles, which are synthesized with FeCl<sub>3</sub> and 2,6-diaminopyrimidine (DAP). These nanoparticles, with an average longitude length of 59.00 nm and a positively charged surface, facilitate the direct delivery of exogenous DNA into the mycelial cells of S. sclerotiorum, as well as successful integration with stable expression. N"],"journal":["Journal of nanobiotechnology"],"pubmed_title":["Fusiform nanoparticle boosts efficient genetic transformation in Sclerotinia sclerotiorum."],"pmcid":["PMC11334516"],"funding_grant_id":["CSTB2023NSCQ-MSX0355","2023YFF1000700","32372077","SWU120075"],"pubmed_authors":["Qian W","Ding Y","Yang N","Lu Y","Rana K","Chen Y","Wan H","Xu J","Xu Z"],"additional_accession":[]},"is_claimable":false,"name":"Fusiform nanoparticle boosts efficient genetic transformation in Sclerotinia sclerotiorum.","description":"<h4>Background</h4>Sclerotinia sclerotiorum is a highly destructive phytopathogenic fungus that poses a significant threat to a wide array of crops. The current constraints in genetic manipulation techniques impede a thorough comprehension of its pathogenic mechanisms and the development of effective control strategies.<h4>Results</h4>Herein, we present a highly efficient genetic transformation system for S. sclerotiorum, leveraging the use of fusiform nanoparticles, which are synthesized with FeCl<sub>3</sub> and 2,6-diaminopyrimidine (DAP). These nanoparticles, with an average longitude length of 59.00 nm and a positively charged surface, facilitate the direct delivery of exogenous DNA into the mycelial cells of S. sclerotiorum, as well as successful integration with stable expression. N","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2026-06-02T04:47:51.981Z","creation":"2025-05-18T12:12:57.436Z"},"accession":"S-EPMC11334516","cross_references":{"pubmed":["39160572"],"doi":["10.1186/s12951-024-02736-6"]}}