biostudies-literatureDing Ythe National Key Research and Development Progragm of ChinaNatural Science Foundation of Chongqingthe Fundamental Research Funds for the Central UniversitiesNational Natural Science Foundation of China494https://www.ebi.ac.uk/biostudies/studies/S-EPMC11334516biostudies-literatureUnknown22(1)<h4>Background</h4>Sclerotinia sclerotiorum is a highly destructive phytopathogenic fungus that poses a significant threat to a wide array of crops. The current constraints in genetic manipulation techniques impede a thorough comprehension of its pathogenic mechanisms and the development of effective control strategies.<h4>Results</h4>Herein, we present a highly efficient genetic transformation system for S. sclerotiorum, leveraging the use of fusiform nanoparticles, which are synthesized with FeCl₃ and 2,6-diaminopyrimidine (DAP). These nanoparticles, with an average longitude length of 59.00 nm and a positively charged surface, facilitate the direct delivery of exogenous DNA into the mycelial cells of S. sclerotiorum, as well as successful integration with stable expression. NJournal of nanobiotechnologyFusiform nanoparticle boosts efficient genetic transformation in Sclerotinia sclerotiorum.PMC11334516CSTB2023NSCQ-MSX03552023YFF100070032372077SWU120075Qian WDing YYang NLu YRana KChen YWan HXu JXu ZfalseFusiform nanoparticle boosts efficient genetic transformation in Sclerotinia sclerotiorum.<h4>Background</h4>Sclerotinia sclerotiorum is a highly destructive phytopathogenic fungus that poses a significant threat to a wide array of crops. The current constraints in genetic manipulation techniques impede a thorough comprehension of its pathogenic mechanisms and the development of effective control strategies.<h4>Results</h4>Herein, we present a highly efficient genetic transformation system for S. sclerotiorum, leveraging the use of fusiform nanoparticles, which are synthesized with FeCl₃ and 2,6-diaminopyrimidine (DAP). These nanoparticles, with an average longitude length of 59.00 nm and a positively charged surface, facilitate the direct delivery of exogenous DNA into the mycelial cells of S. sclerotiorum, as well as successful integration with stable expression. N2024-01-01T00:00:00Z2024 Aug2026-06-02T04:47:51.981Z2025-05-18T12:12:57.436ZS-EPMC113345163916057210.1186/s12951-024-02736-6