biostudies-literatureUnknown14Srinivas N<h4>Background</h4>Mycosis fungoides (MF) is the most common subtype of cutaneous T-cell lymphoma (CTCL). Comprehensive analysis of MF cells <i>in situ</i> and <i>ex vivo</i> is complicated by the fact that is challenging to distinguish malignant from reactive T cells with certainty.<h4>Methods</h4>To overcome this limitation, we performed combined single-cell RNA (scRNAseq) and T-cell receptor TCR sequencing (scTCRseq) of skin lesions of cutaneous MF lesions from 12 patients. A sufficient quantity of living T cells was obtained from 9 patients, but 2 had to be excluded due to unclear diagnoses (coexisting CLL or revision to a fixed toxic drug eruption).<h4>Results</h4>From the remaining patients we established single-cell mRNA expression profiles and the corresponding TCR repertoire of 18Frontiers in oncology1408614https://www.ebi.ac.uk/biostudies/studies/S-EPMC11337020biostudies-literatureSingle-cell RNA and T-cell receptor sequencing unveil mycosis fungoides heterogeneity and a possible gene signature.PMC11337020Farahpour FHorny KSucker ALei KCUgurel SGambichler TKehrmann JPeiffer LSpassova ILuo MSrinivas NBuus TBKubat LLivingstone EYin MOdum NBecker JCfalseSingle-cell RNA and T-cell receptor sequencing unveil mycosis fungoides heterogeneity and a possible gene signature.<h4>Background</h4>Mycosis fungoides (MF) is the most common subtype of cutaneous T-cell lymphoma (CTCL). Comprehensive analysis of MF cells <i>in situ</i> and <i>ex vivo</i> is complicated by the fact that is challenging to distinguish malignant from reactive T cells with certainty.<h4>Methods</h4>To overcome this limitation, we performed combined single-cell RNA (scRNAseq) and T-cell receptor TCR sequencing (scTCRseq) of skin lesions of cutaneous MF lesions from 12 patients. A sufficient quantity of living T cells was obtained from 9 patients, but 2 had to be excluded due to unclear diagnoses (coexisting CLL or revision to a fixed toxic drug eruption).<h4>Results</h4>From the remaining patients we established single-cell mRNA expression profiles and the corresponding TCR repertoire of 182024-01-01T00:00:00Z20242025-04-04T03:10:42.488Z2025-04-04T03:10:42.488ZS-EPMC113370203916994310.3389/fonc.2024.1408614