{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["35(3)"],"submitter":["Kuroda M"],"funding":["National Institute of Biomedical Innovation Health and Nutrition"],"pubmed_abstract":["RNase H-dependent antisense oligonucleotides (gapmer ASOs) represent a class of nucleic acid therapeutics that bind to target RNA to facilitate RNase H-mediated RNA cleavage, thereby regulating the expression of disease-associated proteins. Integrating artificial nucleic acids into gapmer ASOs enhances their therapeutic efficacy. Among these, amido-bridged nucleic acid (AmNA) stands out for its potential to confer high affinity and stability to ASOs. However, a significant challenge in the design of gapmer ASOs incorporating artificial nucleic acids, such as AmNA, is the accurate prediction of their melting temperature (<i>T</i> <sub><i>m</i></sub> ) values. The <i>T</i> <sub><i>m</i></sub> is a critical parameter for designing effective gapmer ASOs to ensure proper functioning. However, p"],"journal":["Molecular therapy. Nucleic acids"],"pagination":["102272"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11339022"],"repository":["biostudies-literature"],"pubmed_title":["Construction of a <i>T</i> <sub>m</sub>-value prediction model and molecular dynamics study of AmNA-containing gapmer antisense oligonucleotide."],"pmcid":["PMC11339022"],"pubmed_authors":["Hirose M","Nagao C","Oda M","Kuroda M","Mizuguchi K","Kasahara Y","Yamaguma H"],"additional_accession":[]},"is_claimable":false,"name":"Construction of a <i>T</i> <sub>m</sub>-value prediction model and molecular dynamics study of AmNA-containing gapmer antisense oligonucleotide.","description":"RNase H-dependent antisense oligonucleotides (gapmer ASOs) represent a class of nucleic acid therapeutics that bind to target RNA to facilitate RNase H-mediated RNA cleavage, thereby regulating the expression of disease-associated proteins. Integrating artificial nucleic acids into gapmer ASOs enhances their therapeutic efficacy. Among these, amido-bridged nucleic acid (AmNA) stands out for its potential to confer high affinity and stability to ASOs. However, a significant challenge in the design of gapmer ASOs incorporating artificial nucleic acids, such as AmNA, is the accurate prediction of their melting temperature (<i>T</i> <sub><i>m</i></sub> ) values. The <i>T</i> <sub><i>m</i></sub> is a critical parameter for designing effective gapmer ASOs to ensure proper functioning. However, p","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2025-04-04T07:55:48.701Z","creation":"2025-04-04T07:55:48.701Z"},"accession":"S-EPMC11339022","cross_references":{"pubmed":["39176173"],"doi":["10.1016/j.omtn.2024.102272"]}}