biostudies-literatureYang Xthe Shenzhen Science and Technology ProgrammeShenzhen Agricultural Development Special Funds (Fishery) Agricultural High-Tech Projectthe National Key Research and Development Programme of Chinathe Natural Science Foundation of Guangdong Provincethe National Key Research and Development Programme371https://www.ebi.ac.uk/biostudies/studies/S-EPMC11355499biostudies-literatureUnknown22(8)<i>Aurantiochytrium</i> is a well-known long-chain polyunsaturated fatty acids (PUFAs) producer, especially docosahexaenoic acid (DHA). In order to reduce the cost or improve the productivity of DHA, many researchers are focusing on exploring the high-yield strain, reducing production costs, changing culture conditions, and other measures. In this study, DHA production was improved by a two-stage fermentation. In the first stage, efficient and cheap soybean powder was used instead of conventional peptone, and the optimization of fermentation conditions (optimal fermentation conditions: temperature 28.7 °C, salinity 10.7‱, nitrogen source concentration 1.01 g/L, and two-nitrogen ratio of yeast extract to soybean powder 2:1) based on response surface methodology resulted in a 1.68-fold increMarine drugsComparative Transcriptomic Analysis on the Effect of Sesamol on the Two-Stages Fermentation of <i>Aurantiochytrium</i> sp. for Enhancing DHA Accumulation.PMC113554992021-9282020YFD09010022021A15150125572018YFA0902500KCXST20221021111206015 and KCXFZ20201221173404012Yang XLiang SLi SWang ZWei LfalseComparative Transcriptomic Analysis on the Effect of Sesamol on the Two-Stages Fermentation of <i>Aurantiochytrium</i> sp. for Enhancing DHA Accumulation.<i>Aurantiochytrium</i> is a well-known long-chain polyunsaturated fatty acids (PUFAs) producer, especially docosahexaenoic acid (DHA). In order to reduce the cost or improve the productivity of DHA, many researchers are focusing on exploring the high-yield strain, reducing production costs, changing culture conditions, and other measures. In this study, DHA production was improved by a two-stage fermentation. In the first stage, efficient and cheap soybean powder was used instead of conventional peptone, and the optimization of fermentation conditions (optimal fermentation conditions: temperature 28.7 °C, salinity 10.7‱, nitrogen source concentration 1.01 g/L, and two-nitrogen ratio of yeast extract to soybean powder 2:1) based on response surface methodology resulted in a 1.68-fold incre2024-01-01T00:00:00Z2024 Aug2025-04-19T20:39:56.508Z2025-04-19T20:39:56.508ZS-EPMC113554993919548710.3390/md22080371