biostudies-literatureYang LChongqing Academy of Agricultural Sciences Core Key Technology Research ProjectChongqing Academy of Agricultural Sciences Performance Incentive Guide ProjectSpecial Funds for Agricultural Development of Chongqing2259https://www.ebi.ac.uk/biostudies/studies/S-EPMC11358934biostudies-literatureUnknown13(16)The benefits of citrus fruits are strongly associated with their secondary metabolites. In this study, we conducted widely targeted metabolomics analyses to compare the variability of the ingredients in four scion-rootstock combinations. A total of 376 differential metabolites were obtained by a multivariate statistical analysis, and a KEGG pathway analysis showed that the enriched metabolic pathways were mainly related to the biosynthesis of flavonoids as well as lipid metabolism. The anthocyanin-targeted metabolomic features showed that cyanidin 3-O-glucoside, cyanidin 3-O-(6-O-malonyl-beta-D-glucoside), cyanidin 3-O-sophoroside, and cyanidin 3-O-xyloside were the pigments responsible for the red color of Tarocco. A lipid metabolomics analysis revealed that when Tarocco was hetero-graftePlants (Basel, Switzerland)Combined Metabolomics and Network Pharmacology Analysis Reveal the Effect of Rootstocks on Anthocyanins, Lipids, and Potential Pharmacological Ingredients of Tarroco Blood Orange (<i>Citrus sinensis</i> L. Osbeck).PMC11358934cqaas2023sjczhx016cqaas2021jxjl01cqaas2023sjczqn013Wang MHong LYang LLi SChen YYu JBai WfalseCombined Metabolomics and Network Pharmacology Analysis Reveal the Effect of Rootstocks on Anthocyanins, Lipids, and Potential Pharmacological Ingredients of Tarroco Blood Orange (<i>Citrus sinensis</i> L. Osbeck).The benefits of citrus fruits are strongly associated with their secondary metabolites. In this study, we conducted widely targeted metabolomics analyses to compare the variability of the ingredients in four scion-rootstock combinations. A total of 376 differential metabolites were obtained by a multivariate statistical analysis, and a KEGG pathway analysis showed that the enriched metabolic pathways were mainly related to the biosynthesis of flavonoids as well as lipid metabolism. The anthocyanin-targeted metabolomic features showed that cyanidin 3-O-glucoside, cyanidin 3-O-(6-O-malonyl-beta-D-glucoside), cyanidin 3-O-sophoroside, and cyanidin 3-O-xyloside were the pigments responsible for the red color of Tarocco. A lipid metabolomics analysis revealed that when Tarocco was hetero-grafte2024-01-01T00:00:00Z2024 Aug2025-05-29T22:16:16.068Z2025-05-29T22:16:16.068ZS-EPMC113589343920469510.3390/plants13162259