biostudies-literatureGarcia-Sobrino RInstituto de Salud Carlos IIIMinisterio de Ciencia, Innovación y UniversidadesAgencia Estatal de Investigación1063https://www.ebi.ac.uk/biostudies/studies/S-EPMC11359487biostudies-literatureUnknown16(8)New clinical strategies for treating severe bone and cartilage injuries are required, especially for use in combination with implant procedures. For this purpose, p(VCL-co-HEMA) thermosensitive hydrogels have been activated with icariin-loaded nanoparticles to be used as bone-cell-harvesting platforms. Supercritical CO₂-SAS technology has been applied to encapsulate icariin, a small molecule that is involved in osteoblastic differentiation. Thus, physical-chemical analysis, including swelling and transmittance, showed the impact of HEMA groups in hydrogel composition. Moreover, icariin (ICA) release from p(VCL-co-HEMA) platforms, including pVCL@ICA nanoparticles, has been studied to evaluate their efficacy in relevant conditions. Finally, the thermosensitive hydrogels' cell compPharmaceuticsOsteoblastic Cell Sheet Engineering Using P(VCL-HEMA)-Based Thermosensitive Hydrogels Doped with pVCL@Icariin Nanoparticles Obtained with Supercritical CO₂-SAS.PMC11359487RTI2018-096328-B-I00PI21/00858PDC2022-133446-I00Reinecke HElvira CGarcia-Crespo AMartinez-Campos ERodriguez-Hernandez JCasado-Losada IGarcia CGarcia-Sobrino RCaltagirone CGallardo AfalseOsteoblastic Cell Sheet Engineering Using P(VCL-HEMA)-Based Thermosensitive Hydrogels Doped with pVCL@Icariin Nanoparticles Obtained with Supercritical CO₂-SAS.New clinical strategies for treating severe bone and cartilage injuries are required, especially for use in combination with implant procedures. For this purpose, p(VCL-co-HEMA) thermosensitive hydrogels have been activated with icariin-loaded nanoparticles to be used as bone-cell-harvesting platforms. Supercritical CO₂-SAS technology has been applied to encapsulate icariin, a small molecule that is involved in osteoblastic differentiation. Thus, physical-chemical analysis, including swelling and transmittance, showed the impact of HEMA groups in hydrogel composition. Moreover, icariin (ICA) release from p(VCL-co-HEMA) platforms, including pVCL@ICA nanoparticles, has been studied to evaluate their efficacy in relevant conditions. Finally, the thermosensitive hydrogels' cell comp2024-01-01T00:00:00Z2024 Aug2025-04-04T10:38:56.37Z2024-10-16T14:29:23.649ZS-EPMC113594873920440810.3390/pharmaceutics16081063