biostudies-literatureYing SQYoung Science and Technology Rising Star Project of Shaanxi ProvinceNational Natural Science Foundation of ChinaChina Postdoctoral Science Foundation521https://www.ebi.ac.uk/biostudies/studies/S-EPMC11363633biostudies-literatureUnknown22(1)Tissue-derived extracellular vesicles (EVs) are emerging as pivotal players to maintain organ homeostasis, which show promise as a next-generation candidate for medical use with extensive source. However, the detailed function and therapeutic potential of tissue EVs remain insufficiently studied. Here, through bulk and single-cell RNA sequencing analyses combined with ultrastructural tissue examinations, we first reveal that in situ liver tissue EVs (LT-EVs) contribute to the intricate liver regenerative process after partial hepatectomy (PHx), and that hepatocytes are the primary source of tissue EVs in the regenerating liver. Nanoscale and proteomic profiling further identify that the hepatocyte-specific tissue EVs (Hep-EVs) are strengthened to release with carrying proliferative messageJournal of nanobiotechnologyHepatocyte-derived tissue extracellular vesicles safeguard liver regeneration and support regenerative therapy.PMC11363633BX202304858237102082301028819300252023KJXX-02782170988Ying SQXing SJZhang KZhou ZKQiu JYJin YZheng CXLuo XYZhang XHJin FCao YSui BDLi YYShi KfalseHepatocyte-derived tissue extracellular vesicles safeguard liver regeneration and support regenerative therapy.Tissue-derived extracellular vesicles (EVs) are emerging as pivotal players to maintain organ homeostasis, which show promise as a next-generation candidate for medical use with extensive source. However, the detailed function and therapeutic potential of tissue EVs remain insufficiently studied. Here, through bulk and single-cell RNA sequencing analyses combined with ultrastructural tissue examinations, we first reveal that in situ liver tissue EVs (LT-EVs) contribute to the intricate liver regenerative process after partial hepatectomy (PHx), and that hepatocytes are the primary source of tissue EVs in the regenerating liver. Nanoscale and proteomic profiling further identify that the hepatocyte-specific tissue EVs (Hep-EVs) are strengthened to release with carrying proliferative message2024-01-01T00:00:00Z2024 Aug2026-05-02T03:39:26.194Z2025-04-05T12:30:52.245ZS-EPMC113636333921034610.1186/s12951-024-02790-0