biostudies-literatureSun MNational Natural Science Foundation of ChinaThe Key Science &amp; Technology Project of GEM Flower Healthcare2391521https://www.ebi.ac.uk/biostudies/studies/S-EPMC11364064biostudies-literatureUnknown16(1)N-Nitrosamine disinfection by-products (NAs-DBPs) have been well proven for its role in esophageal carcinogenesis. However, the role of intratumoral microorganisms in esophageal squamous cell carcinoma (ESCC) has not yet been well explored in the context of exposure to NAs-DBPs. Here, the multi-omics integration reveals <i>F. periodonticum</i> (<i>Fp</i>) as "facilitators" is highly enriched in cancer tissues and promotes the epithelial mesenchymal transition (EMT)-like subtype formation of ESCC. We demonstrate that <i>Fp</i> potently drives de novo synthesis of fatty acids, migration, invasion and EMT phenotype through its unique FadAL adhesin. However, N-nitrosomethylbenzylamine upregulates the transcription level of FadAL. Mechanistically, co-immunoprecipitation coupled to mass spectromGut microbesSynergism of Fusobacterium periodonticum and N-nitrosamines promote the formation of EMT subtypes in ESCC by modulating Wnt3a palmitoylation.PMC11364064GTBSH202482173479Sun MLiao YLin QHuang YYe PGuo XHu MLiu RPeng ZShen WfalseSynergism of Fusobacterium periodonticum and N-nitrosamines promote the formation of EMT subtypes in ESCC by modulating Wnt3a palmitoylation.N-Nitrosamine disinfection by-products (NAs-DBPs) have been well proven for its role in esophageal carcinogenesis. However, the role of intratumoral microorganisms in esophageal squamous cell carcinoma (ESCC) has not yet been well explored in the context of exposure to NAs-DBPs. Here, the multi-omics integration reveals <i>F. periodonticum</i> (<i>Fp</i>) as "facilitators" is highly enriched in cancer tissues and promotes the epithelial mesenchymal transition (EMT)-like subtype formation of ESCC. We demonstrate that <i>Fp</i> potently drives de novo synthesis of fatty acids, migration, invasion and EMT phenotype through its unique FadAL adhesin. However, N-nitrosomethylbenzylamine upregulates the transcription level of FadAL. Mechanistically, co-immunoprecipitation coupled to mass spectrom2024-01-01T00:00:00Z2024 Jan-Dec2026-04-13T03:13:51.693Z2024-10-16T14:30:45.64ZS-EPMC113640643919361810.1080/19490976.2024.2391521