biostudies-literatureGarg RNational Institute of Neurological Disorders and StrokeNational Center for Research ResourcesNCRR NIH HHSNational Institutes of HealthNational Institute of General Medical SciencesNIGMS NIH HHSNational Science Foundation107726https://www.ebi.ac.uk/biostudies/studies/S-EPMC11364148biostudies-literatureUnknown365Improving the spectral sensitivity and resolution of biological solids is one of the long-standing problems in nuclear magnetic resonance (NMR) spectroscopy. In this report, we introduce low-power supercycled variants of two-pulse phase-modulated (TPPM) sequence for heteronuclear decoupling. The utility of the sequence is shown by improvements in the transverse relaxation time of observed nuclei (with ¹H decoupling) with its application to different samples (uniformly ¹³C, ¹⁵N, ²H-labeled GB1 back-exchanged with 25% H₂O and 75% D₂O, uniformly ¹³C, ¹⁵N, ²H-labeled human derived Asyn fibril back-exchanged with 100% H₂O and uniformly ¹³C, ¹⁵N -labeled human derivJournal of magnetic resonance (San Diego, Calif. : 1997)Low power supercycled TPPM decoupling.PMC11364148DBI 1946970P41GM136463S10RR025037P41 GM136463S10 RR025037Garg RRienstra CMDeZonia BPaterson ALfalseLow power supercycled TPPM decoupling.Improving the spectral sensitivity and resolution of biological solids is one of the long-standing problems in nuclear magnetic resonance (NMR) spectroscopy. In this report, we introduce low-power supercycled variants of two-pulse phase-modulated (TPPM) sequence for heteronuclear decoupling. The utility of the sequence is shown by improvements in the transverse relaxation time of observed nuclei (with ¹H decoupling) with its application to different samples (uniformly ¹³C, ¹⁵N, ²H-labeled GB1 back-exchanged with 25% H₂O and 75% D₂O, uniformly ¹³C, ¹⁵N, ²H-labeled human derived Asyn fibril back-exchanged with 100% H₂O and uniformly ¹³C, ¹⁵N -labeled human deriv2024-01-01T00:00:00Z2024 Aug2026-03-18T13:25:36.69Z2025-08-18T09:53:54.471ZS-EPMC113641483899126710.1016/j.jmr.2024.107726