biostudies-literatureUnknown15Juli MSB<h4>Background</h4>Bovine genital campylobacteriosis (BGC) is a globally important venereal disease of cattle caused by <i>Campylobacter fetus</i> subspecies <i>venerealis</i>. Diagnosis of BGC is highly challenging due to the lack of accurate diagnostic tests.<h4>Methods</h4>To characterise the biomarkers for <i>C. fetus venerealis</i> infection, a total of twelve cycling heifers were selected and categorised as vaccinated (<i>n</i> = 6) with Vibrovax® (Zoetis™) and unvaccinated (<i>n</i> = 6). All heifers were oestrous synchronised with a double dose of prostaglandin (PGF2α) 11 days apart and when in oestrous intravaginally challenged with 2.7 x 10⁹ CFU live <i>C. fetus venerealis</i>. DNA extracted from vaginal mucus samples was screened using a <i>C. fetus</i> qPCR and 16S rFrontiers in microbiology1404525https://www.ebi.ac.uk/biostudies/studies/S-EPMC11366586biostudies-literatureCharacterisation of reproductive tract microbiome and immune biomarkers for bovine genital campylobacteriosis in vaccinated and unvaccinated heifers.PMC11366586Siddle HVBoe-Hansen GBForutan MJuli MSBTabor AERaza AFordyce GMuller JfalseCharacterisation of reproductive tract microbiome and immune biomarkers for bovine genital campylobacteriosis in vaccinated and unvaccinated heifers.<h4>Background</h4>Bovine genital campylobacteriosis (BGC) is a globally important venereal disease of cattle caused by <i>Campylobacter fetus</i> subspecies <i>venerealis</i>. Diagnosis of BGC is highly challenging due to the lack of accurate diagnostic tests.<h4>Methods</h4>To characterise the biomarkers for <i>C. fetus venerealis</i> infection, a total of twelve cycling heifers were selected and categorised as vaccinated (<i>n</i> = 6) with Vibrovax® (Zoetis™) and unvaccinated (<i>n</i> = 6). All heifers were oestrous synchronised with a double dose of prostaglandin (PGF2α) 11 days apart and when in oestrous intravaginally challenged with 2.7 x 10⁹ CFU live <i>C. fetus venerealis</i>. DNA extracted from vaginal mucus samples was screened using a <i>C. fetus</i> qPCR and 16S r2024-01-01T00:00:00Z20242026-06-01T16:15:06.849Z2024-11-15T04:43:34.081ZS-EPMC113665863922421910.3389/fmicb.2024.1404525