biostudies-literatureUnknown14(1)Liu DSecondary BRAF variations have been identified as a mechanism of resistance to tyrosine kinase inhibitors (TKIs) in patients with driver gene-positive NSCLC. Nevertheless, there is still a lack of consensus regarding the characteristics and subsequent treatment strategies for these patients. We retrospectively reviewed the medical records of patients with driver gene-positive NSCLC who received TKIs therapy at Zhejiang Cancer Hospital between May 2016 and December 2023. The clinical and genetic characteristics of these patients were assessed, along with the impact of various treatment strategies on survival. This study enrolled 27 patients with advanced NSCLC, in whom BRAF variations occurred at a median time of 28 months after the initiation of targeted therapy. The multivariate acceleratScientific reports20302https://www.ebi.ac.uk/biostudies/studies/S-EPMC11366755biostudies-literatureA real world analysis of secondary BRAF variations after targeted therapy resistance in driver gene positive NSCLC.PMC11366755Li XPan YJin XLiu DDing KYin KPeng ZXu YfalseA real world analysis of secondary BRAF variations after targeted therapy resistance in driver gene positive NSCLC.Secondary BRAF variations have been identified as a mechanism of resistance to tyrosine kinase inhibitors (TKIs) in patients with driver gene-positive NSCLC. Nevertheless, there is still a lack of consensus regarding the characteristics and subsequent treatment strategies for these patients. We retrospectively reviewed the medical records of patients with driver gene-positive NSCLC who received TKIs therapy at Zhejiang Cancer Hospital between May 2016 and December 2023. The clinical and genetic characteristics of these patients were assessed, along with the impact of various treatment strategies on survival. This study enrolled 27 patients with advanced NSCLC, in whom BRAF variations occurred at a median time of 28 months after the initiation of targeted therapy. The multivariate accelerat2024-01-01T00:00:00Z2024 Sep2025-04-05T12:29:38.029Z2025-04-05T12:29:38.029ZS-EPMC113667553921891910.1038/s41598-024-71143-6