{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chou CY"],"funding":["National Taiwan University","Reliance Biosciences Inc."],"pagination":["44386-44398"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11367580"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(34)"],"pubmed_abstract":["Extracellular vesicles (EVs) are present in blood at much lower concentrations (5-6 orders of magnitude) compared to lipoprotein particles (LP). Because LP and EV overlap in size and density, isolating high-purity EVs is a significant challenge. While the current two-step sequential EV isolation process using size-expression chromatography (SEC) followed by a density gradient (DG) achieves high purity, the time-consuming ultracentrifugation (UC) step in DG hinders workflow efficiency. This paper introduces an optimized magnetic bead reagent, LipoMin, functionalized with glycosaminoglycans (GAGs), as a rapid alternative for LP removal during the second-step process in about 10 minutes. We evaluated LipoMin's efficacy on two sample types: (a) EV fractions isolated by size exclusion chromatog"],"journal":["ACS applied materials & interfaces"],"pubmed_title":["Improving the Purity of Extracellular Vesicles by Removal of Lipoproteins from Size Exclusion Chromatography- and Ultracentrifugation-Processed Samples Using Glycosaminoglycan-Functionalized Magnetic Beads."],"pmcid":["PMC11367580"],"funding_grant_id":["2022-03-001","112HT549002"],"pubmed_authors":["Chou CY","Chang JW","Hsu WF","Wu MS","Chiang PC","Chang LC","Wo AM","Lu PH","Li CC","Hsu JL"],"additional_accession":[]},"is_claimable":false,"name":"Improving the Purity of Extracellular Vesicles by Removal of Lipoproteins from Size Exclusion Chromatography- and Ultracentrifugation-Processed Samples Using Glycosaminoglycan-Functionalized Magnetic Beads.","description":"Extracellular vesicles (EVs) are present in blood at much lower concentrations (5-6 orders of magnitude) compared to lipoprotein particles (LP). Because LP and EV overlap in size and density, isolating high-purity EVs is a significant challenge. While the current two-step sequential EV isolation process using size-expression chromatography (SEC) followed by a density gradient (DG) achieves high purity, the time-consuming ultracentrifugation (UC) step in DG hinders workflow efficiency. This paper introduces an optimized magnetic bead reagent, LipoMin, functionalized with glycosaminoglycans (GAGs), as a rapid alternative for LP removal during the second-step process in about 10 minutes. We evaluated LipoMin's efficacy on two sample types: (a) EV fractions isolated by size exclusion chromatog","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2025-04-26T11:43:30.528Z","creation":"2025-04-06T13:45:33.451Z"},"accession":"S-EPMC11367580","cross_references":{"pubmed":["39149774"],"doi":["10.1021/acsami.4c03869"]}}