{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Cisneros-Aguirre M"],"funding":["NCI NIH HHS"],"pubmed_abstract":["A key step of Canonical Nonhomologous End Joining (C-NHEJ) is synapsis of DNA double strand break (DSB) ends for ligation. The DNA-PKcs dimer mediates synapsis in a long-range complex with DSB ends remaining apart, whereas the XLF homodimer can mediate synapsis in both long-range and short-range complexes. Recent structural studies found the PAXX homodimer may also facilitate synapsis in long-range complexes with DNA-PKcs via its interactions with Ku70. Thus, we examined the influence of PAXX in C-NHEJ of chromosomal DSBs, which we compared to another Ku-binding factor, MRI. Using EJ of blunt DSBs with Cas9 reporters as a readout for C-NHEJ, we found that PAXX and/or MRI are dispensable. However, when combined with disruption of DNA-PKcs, particularly with DNA-PKcs kinase inhibition, PAXX "],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.08.21.607864"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11370355"],"repository":["biostudies-literature"],"pubmed_title":["Distinct functions of PAXX and MRI during chromosomal end joining."],"pmcid":["PMC11370355"],"funding_grant_id":["R01 CA240392","P30 CA033572","F99 CA284248","R01 CA256989"],"pubmed_authors":["Ping X","Cisneros-Aguirre M","Lopezcolorado FW","Stark JM","Chen R"],"additional_accession":[]},"is_claimable":false,"name":"Distinct functions of PAXX and MRI during chromosomal end joining.","description":"A key step of Canonical Nonhomologous End Joining (C-NHEJ) is synapsis of DNA double strand break (DSB) ends for ligation. The DNA-PKcs dimer mediates synapsis in a long-range complex with DSB ends remaining apart, whereas the XLF homodimer can mediate synapsis in both long-range and short-range complexes. Recent structural studies found the PAXX homodimer may also facilitate synapsis in long-range complexes with DNA-PKcs via its interactions with Ku70. Thus, we examined the influence of PAXX in C-NHEJ of chromosomal DSBs, which we compared to another Ku-binding factor, MRI. Using EJ of blunt DSBs with Cas9 reporters as a readout for C-NHEJ, we found that PAXX and/or MRI are dispensable. However, when combined with disruption of DNA-PKcs, particularly with DNA-PKcs kinase inhibition, PAXX ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2025-04-04T02:57:41.575Z","creation":"2025-04-04T02:57:41.575Z"},"accession":"S-EPMC11370355","cross_references":{"pubmed":["39229097"],"doi":["10.1101/2024.08.21.607864"]}}