{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Otomo A"],"funding":["NIGMS NIH HHS"],"pubmed_abstract":["Understanding the function of rotary molecular motors, such as the rotary ATPases, relies on our ability to visualize the single-molecule rotation. Traditional imaging methods often involve tagging those motors with nanoparticles (NPs) and inferring their rotation from translational motion of NPs. Here, we report an approach using \"two-faced\" Janus NPs to directly image the rotation of single V-ATPase from <i>Enterococcus hirae</i>, an ATP-driven rotary ion pump. By employing a 500-nm silica/gold Janus NP, we exploit its asymmetric optical contrast - silica core with a gold cap on one hemisphere - to achieve precise imaging of the unidirectional counter-clockwise rotation of single V-ATPase motors immobilized on surfaces. Despite the added viscous load from the relatively large Janus NP pr"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.08.22.609254"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11370591"],"repository":["biostudies-literature"],"pubmed_title":["Visualizing Single V-ATPase Rotation Using Janus Nanoparticles."],"pmcid":["PMC11370591"],"funding_grant_id":["R35 GM124918"],"pubmed_authors":["Otomo A","Yamamoto M","Bhattacharyya S","Wiemann J","Iino R","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"Visualizing Single V-ATPase Rotation Using Janus Nanoparticles.","description":"Understanding the function of rotary molecular motors, such as the rotary ATPases, relies on our ability to visualize the single-molecule rotation. Traditional imaging methods often involve tagging those motors with nanoparticles (NPs) and inferring their rotation from translational motion of NPs. Here, we report an approach using \"two-faced\" Janus NPs to directly image the rotation of single V-ATPase from <i>Enterococcus hirae</i>, an ATP-driven rotary ion pump. By employing a 500-nm silica/gold Janus NP, we exploit its asymmetric optical contrast - silica core with a gold cap on one hemisphere - to achieve precise imaging of the unidirectional counter-clockwise rotation of single V-ATPase motors immobilized on surfaces. Despite the added viscous load from the relatively large Janus NP pr","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2025-04-04T02:54:36.616Z","creation":"2025-04-04T02:54:36.616Z"},"accession":"S-EPMC11370591","cross_references":{"pubmed":["39229122"],"doi":["10.1101/2024.08.22.609254"]}}