biostudies-literatureUnknown1210Fang XTwo new species, Macropelopia (Macropelopia) excavata Xu & Fu, <b>sp. nov.</b> and Macropelopia (Macropelopia) quadrimacula Xu & Fu, <b>sp. nov.</b>, are described as male adults. A key to identify the males of <i>Macropelopia</i> from China is provided. Furthermore, in order to ascertain the genetic distance between these species and their morphological characteristics, mitochondrial cytochrome <i>c</i> oxidase subunit I gene sequences were uploaded to the National Center for Biotechnology Information. These COI sequences were then utilized to infer the relationships between the species, employing the neighbor-joining method.ZooKeys287-298https://www.ebi.ac.uk/biostudies/studies/S-EPMC11372292biostudies-literatureTwo new species of <i>Macropelopia</i> (Diptera, Chironomidae) from Oriental China, delineated with morphology and COI sequences.PMC11372292Fang XYao YFu YXu ZfalseTwo new species of <i>Macropelopia</i> (Diptera, Chironomidae) from Oriental China, delineated with morphology and COI sequences.Two new species, Macropelopia (Macropelopia) excavata Xu & Fu, <b>sp. nov.</b> and Macropelopia (Macropelopia) quadrimacula Xu & Fu, <b>sp. nov.</b>, are described as male adults. A key to identify the males of <i>Macropelopia</i> from China is provided. Furthermore, in order to ascertain the genetic distance between these species and their morphological characteristics, mitochondrial cytochrome <i>c</i> oxidase subunit I gene sequences were uploaded to the National Center for Biotechnology Information. These COI sequences were then utilized to infer the relationships between the species, employing the neighbor-joining method.2024-01-01T00:00:00Z20242025-04-22T07:41:04.379Z2025-04-05T22:14:30.22ZS-EPMC113722923923415010.3897/zookeys.1210.127183