biostudies-literatureUnknown9(35)Zhang SThe biosensing field faces a significant challenge in efficiently detecting multiple analytes in a single diagnostic sample in order to compete with other established multiplex molecular diagnostic technologies such as PCR and ELISA. In response, we have developed a colorimetric nanobiosensor based on multiple morphological forms of functionalized gold nanoparticles (AuNPs) for the simultaneous detection of the influenza virus and SARS-CoV-2 virus. Gold nanospheres (GNSp) were modified with oligonucleotides specific for the influenza A virus, while gold nanoshells (GNSh) were modified with oligonucleotides specific for the SARS-CoV-2 virus. In the presence of their respective targets, AuNPs remain stable due to DNA-DNA interactions; conversely, in the absence of targets, AuNPs aggregate. CACS omega37163-37169https://www.ebi.ac.uk/biostudies/studies/S-EPMC11375896biostudies-literatureShape-Specific Gold Nanoparticles for Multiplex Biosensing Applications.PMC11375896Jiang JCharconnet MPeng YZhang YZhang LZhang SLawrie CHfalseShape-Specific Gold Nanoparticles for Multiplex Biosensing Applications.The biosensing field faces a significant challenge in efficiently detecting multiple analytes in a single diagnostic sample in order to compete with other established multiplex molecular diagnostic technologies such as PCR and ELISA. In response, we have developed a colorimetric nanobiosensor based on multiple morphological forms of functionalized gold nanoparticles (AuNPs) for the simultaneous detection of the influenza virus and SARS-CoV-2 virus. Gold nanospheres (GNSp) were modified with oligonucleotides specific for the influenza A virus, while gold nanoshells (GNSh) were modified with oligonucleotides specific for the SARS-CoV-2 virus. In the presence of their respective targets, AuNPs remain stable due to DNA-DNA interactions; conversely, in the absence of targets, AuNPs aggregate. C2024-01-01T00:00:00Z2024 Sep2025-04-19T12:40:49.185Z2025-04-19T12:40:49.185ZS-EPMC113758963924646810.1021/acsomega.4c04385