biostudies-literatureFlorczyk SJNCRR NIH HHSIntramural NIST DOCU.S. Department of CommerceNational Science Foundation2302-2313https://www.ebi.ac.uk/biostudies/studies/S-EPMC11376592biostudies-literatureUnknown3(10)Many biomaterial scaffolds have been advanced to provide synthetic cell niches for tissue engineering and drug screening applications; however, current methods for comparing scaffold niches focus on cell functional outcomes or attempt to normalize materials properties between different scaffold formats. We demonstrate a three-dimensional (3D) cellular morphotyping strategy for comparing biomaterial scaffold cell niches between different biomaterial scaffold formats. Primary human bone marrow stromal cells (hBMSCs) were cultured on 8 different biomaterial scaffolds, including fibrous scaffolds, hydrogels, and porous sponges, in 10 treatment groups to compare a variety of biomaterial scaffolds and cell morphologies. A bioinformatics approach was used to determine the 3D cellular morphotype fACS biomaterials science & engineeringA Bioinformatics 3D Cellular Morphotyping Strategy for Assessing Biomaterial Scaffold Niches.PMC11376592P40 RR0174479999-NISTPHY1205965Simon CGCardone ABrady MCSarkar SBodhak SSimon MBaker PJPine PSBajcsy PJuba DFlorczyk SJChen DfalseA Bioinformatics 3D Cellular Morphotyping Strategy for Assessing Biomaterial Scaffold Niches.Many biomaterial scaffolds have been advanced to provide synthetic cell niches for tissue engineering and drug screening applications; however, current methods for comparing scaffold niches focus on cell functional outcomes or attempt to normalize materials properties between different scaffold formats. We demonstrate a three-dimensional (3D) cellular morphotyping strategy for comparing biomaterial scaffold cell niches between different biomaterial scaffold formats. Primary human bone marrow stromal cells (hBMSCs) were cultured on 8 different biomaterial scaffolds, including fibrous scaffolds, hydrogels, and porous sponges, in 10 treatment groups to compare a variety of biomaterial scaffolds and cell morphologies. A bioinformatics approach was used to determine the 3D cellular morphotype f2017-01-01T00:00:00Z2017 Oct2026-05-08T20:05:54.171Z2025-04-05T14:38:52.756ZS-EPMC113765923344528910.1021/acsbiomaterials.7b00473