{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["27(9)"],"submitter":["Yamazaki T"],"pubmed_abstract":["Detecting antibodies, particularly those targeting donor human leukocyte antigens in organ transplantation and self-antigens in autoimmune diseases, is crucial for diagnosis and therapy. Radioprotective 105 (RP105), a Toll-like receptor family protein, is expressed in immune-competent cells, such as B cells. Studies in mice have shown that the anti-mouse RP105 antibody strongly activates B cells and triggers an adjuvant effect against viral infections. However, the anti-human RP105 antibody (ɑhRP105) weakly activates human B cells. This study established new culture conditions under, which human B cells are strongly activated by the ɑhRP105. When combined with CpGDNA, specific antibody production against blood group carbohydrates, ɑGal, and SARS-CoV-2 was successfully detected in human B c"],"journal":["iScience"],"pagination":["110649"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11380396"],"repository":["biostudies-literature"],"pubmed_title":["Human RP105 monoclonal antibody enhances antigen-specific antibody production in unique culture conditions."],"pmcid":["PMC11380396"],"pubmed_authors":["Imai M","Okada M","Kobayashi T","Ishiyama K","Shizuku M","Akashi-Takamura S","Okuzaki D","Inui M","Tomono S","Iwasaki K","Miwa Y","Yamazaki T","Ashimine S"],"additional_accession":[]},"is_claimable":false,"name":"Human RP105 monoclonal antibody enhances antigen-specific antibody production in unique culture conditions.","description":"Detecting antibodies, particularly those targeting donor human leukocyte antigens in organ transplantation and self-antigens in autoimmune diseases, is crucial for diagnosis and therapy. Radioprotective 105 (RP105), a Toll-like receptor family protein, is expressed in immune-competent cells, such as B cells. Studies in mice have shown that the anti-mouse RP105 antibody strongly activates B cells and triggers an adjuvant effect against viral infections. However, the anti-human RP105 antibody (ɑhRP105) weakly activates human B cells. This study established new culture conditions under, which human B cells are strongly activated by the ɑhRP105. When combined with CpGDNA, specific antibody production against blood group carbohydrates, ɑGal, and SARS-CoV-2 was successfully detected in human B c","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-06-02T22:58:58.436Z","creation":"2025-04-04T00:26:20.716Z"},"accession":"S-EPMC11380396","cross_references":{"pubmed":["39246445"],"doi":["10.1016/j.isci.2024.110649"]}}