biostudies-literatureUnknown27(9)Yamazaki TDetecting antibodies, particularly those targeting donor human leukocyte antigens in organ transplantation and self-antigens in autoimmune diseases, is crucial for diagnosis and therapy. Radioprotective 105 (RP105), a Toll-like receptor family protein, is expressed in immune-competent cells, such as B cells. Studies in mice have shown that the anti-mouse RP105 antibody strongly activates B cells and triggers an adjuvant effect against viral infections. However, the anti-human RP105 antibody (ɑhRP105) weakly activates human B cells. This study established new culture conditions under, which human B cells are strongly activated by the ɑhRP105. When combined with CpGDNA, specific antibody production against blood group carbohydrates, ɑGal, and SARS-CoV-2 was successfully detected in human B ciScience110649https://www.ebi.ac.uk/biostudies/studies/S-EPMC11380396biostudies-literatureHuman RP105 monoclonal antibody enhances antigen-specific antibody production in unique culture conditions.PMC11380396Imai MOkada MKobayashi TIshiyama KShizuku MAkashi-Takamura SOkuzaki DInui MTomono SIwasaki KMiwa YYamazaki TAshimine SfalseHuman RP105 monoclonal antibody enhances antigen-specific antibody production in unique culture conditions.Detecting antibodies, particularly those targeting donor human leukocyte antigens in organ transplantation and self-antigens in autoimmune diseases, is crucial for diagnosis and therapy. Radioprotective 105 (RP105), a Toll-like receptor family protein, is expressed in immune-competent cells, such as B cells. Studies in mice have shown that the anti-mouse RP105 antibody strongly activates B cells and triggers an adjuvant effect against viral infections. However, the anti-human RP105 antibody (ɑhRP105) weakly activates human B cells. This study established new culture conditions under, which human B cells are strongly activated by the ɑhRP105. When combined with CpGDNA, specific antibody production against blood group carbohydrates, ɑGal, and SARS-CoV-2 was successfully detected in human B c2024-01-01T00:00:00Z2024 Sep2026-06-02T22:58:58.436Z2025-04-04T00:26:20.716ZS-EPMC113803963924644510.1016/j.isci.2024.110649