{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Lim G"],"pubmed_abstract":["Despite the increasing demand for efficient and sustainable chemical processes, the development of scalable systems using biocatalysis for fine chemical production remains a significant challenge. We have developed a scalable flow system using immobilized enzymes to facilitate flavin-dependent biocatalysis, targeting as a proof-of-concept asymmetric alkene reduction. The system integrates a flavin-dependent Old Yellow Enzyme (OYE) and a soluble hydrogenase to enable H<sub>2</sub>-driven regeneration of the OYE cofactor FMNH<sub>2</sub>. Molecular hydrogen was produced by water electrolysis using a proton exchange membrane (PEM) electrolyzer and introduced into the flow system via a designed gas membrane addition module at a high diffusion rate. The flow system shows remarkable stability an"],"journal":["Communications chemistry"],"pagination":["200"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11380674"],"repository":["biostudies-literature"],"pubmed_title":["H<sub>2</sub>-driven biocatalysis for flavin-dependent ene-reduction in a continuous closed-loop flow system utilizing H<sub>2</sub> from water electrolysis."],"pmcid":["PMC11380674"],"pubmed_authors":["Paul CE","Cordero PRF","Mulks FF","Lauterbach L","Lim G","Calabrese D","Wolder A","Rother D"],"additional_accession":[]},"is_claimable":false,"name":"H<sub>2</sub>-driven biocatalysis for flavin-dependent ene-reduction in a continuous closed-loop flow system utilizing H<sub>2</sub> from water electrolysis.","description":"Despite the increasing demand for efficient and sustainable chemical processes, the development of scalable systems using biocatalysis for fine chemical production remains a significant challenge. We have developed a scalable flow system using immobilized enzymes to facilitate flavin-dependent biocatalysis, targeting as a proof-of-concept asymmetric alkene reduction. The system integrates a flavin-dependent Old Yellow Enzyme (OYE) and a soluble hydrogenase to enable H<sub>2</sub>-driven regeneration of the OYE cofactor FMNH<sub>2</sub>. Molecular hydrogen was produced by water electrolysis using a proton exchange membrane (PEM) electrolyzer and introduced into the flow system via a designed gas membrane addition module at a high diffusion rate. The flow system shows remarkable stability an","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2025-04-18T21:42:10.994Z","creation":"2025-02-19T02:33:59.804Z"},"accession":"S-EPMC11380674","cross_references":{"pubmed":["39244618"],"doi":["10.1038/s42004-024-01288-y"]}}