{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li M"],"funding":["Anhui Science and Technology Department (Department of Science and Technology of Anhui Province)","National Natural Science Foundation of China (National Science Foundation of China)"],"pagination":["1100"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11380689"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(1)"],"pubmed_abstract":["PHD2 is essential in modulating HIF-1α levels upon oxygen fluctuations. Hypoxia, a hallmark of uterus, and HIF-1α have recently emerged as opposing regulators of mesendoderm specification, suggesting a role for PHD2 therein. We found that PHD2 expression initially covered the epiblast and gradually receded from the primitive streak, which was identical to hypoxia and exclusive to HIF-1α. The investigations performed in mESCs, embryoids, and mouse embryos together demonstrated that PHD2 negatively regulated mesendoderm specification. Single-cell RNA sequencing revealed that PHD2 governed the transition from epiblast to mesendoderm. The downstream effect of PHD2 relied on the HIF-1α regulated Wnt/β-catenin pathway, while it was regulated upstream by miR-429. In summary, our research highligh"],"journal":["Communications biology"],"pubmed_title":["PHD2 safeguards modest mesendoderm development."],"pmcid":["PMC11380689"],"funding_grant_id":["2208085MH209, 1808085QH234","31701289"],"pubmed_authors":["Liu Y","Jin H","Long H","Zhu G","Shen X","Li M","Zhao Y"],"additional_accession":[]},"is_claimable":false,"name":"PHD2 safeguards modest mesendoderm development.","description":"PHD2 is essential in modulating HIF-1α levels upon oxygen fluctuations. Hypoxia, a hallmark of uterus, and HIF-1α have recently emerged as opposing regulators of mesendoderm specification, suggesting a role for PHD2 therein. We found that PHD2 expression initially covered the epiblast and gradually receded from the primitive streak, which was identical to hypoxia and exclusive to HIF-1α. The investigations performed in mESCs, embryoids, and mouse embryos together demonstrated that PHD2 negatively regulated mesendoderm specification. Single-cell RNA sequencing revealed that PHD2 governed the transition from epiblast to mesendoderm. The downstream effect of PHD2 relied on the HIF-1α regulated Wnt/β-catenin pathway, while it was regulated upstream by miR-429. In summary, our research highligh","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-05-28T01:42:11.174Z","creation":"2025-04-21T17:02:52.589Z"},"accession":"S-EPMC11380689","cross_references":{"pubmed":["39244636"],"doi":["10.1038/s42003-024-06824-z"]}}