{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liegeois MA"],"funding":["National Science Foundation Graduate Research Fellowship Program","NIAID NIH HHS","NHLBI NIH HHS","NIAID","NIH HHS","Cystic Fibrosis Trust","NIH National Heart Lung and Blood Institute"],"pagination":["e181024"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11383604"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(15)"],"pubmed_abstract":["Mucus plugs occlude airways to obstruct airflow in asthma. Studies in patients and in mouse models show that mucus plugs occur in the context of type 2 inflammation, and studies in human airway epithelial cells (HAECs) show that IL-13-activated cells generate pathologic mucus independently of immune cells. To determine how HAECs autonomously generate pathologic mucus, we used a magnetic microwire rheometer to characterize the viscoelastic properties of mucus secreted under varying conditions. We found that normal HAEC mucus exhibited viscoelastic liquid behavior and that mucus secreted by IL-13-activated HAECs exhibited solid-like behavior caused by mucin cross-linking. In addition, IL-13-activated HAECs shows increased peroxidase activity in apical secretions, and an overlaid thiolated po"],"journal":["JCI insight"],"pubmed_title":["Peroxidase-mediated mucin cross-linking drives pathologic mucus gel formation in IL-13-stimulated airway epithelial cells."],"pmcid":["PMC11383604"],"funding_grant_id":["NA","U19 AI077439","P01 HL107202","R01 HL080414","HL080414,HL107202","S10 OD028611","R01 HL164787","AI077439"],"pubmed_authors":["Moore WC","Wenzel SE","Braunreuther M","Castro M","Woodruff PG","Fuller GG","Fahy JV","Charbit AR","Levy BD","Raymond WW","Jarjour NN","Tang M","Israel E","Erzurum SC","Liegeois MA","Christenson SA"],"additional_accession":[]},"is_claimable":false,"name":"Peroxidase-mediated mucin cross-linking drives pathologic mucus gel formation in IL-13-stimulated airway epithelial cells.","description":"Mucus plugs occlude airways to obstruct airflow in asthma. Studies in patients and in mouse models show that mucus plugs occur in the context of type 2 inflammation, and studies in human airway epithelial cells (HAECs) show that IL-13-activated cells generate pathologic mucus independently of immune cells. To determine how HAECs autonomously generate pathologic mucus, we used a magnetic microwire rheometer to characterize the viscoelastic properties of mucus secreted under varying conditions. We found that normal HAEC mucus exhibited viscoelastic liquid behavior and that mucus secreted by IL-13-activated HAECs exhibited solid-like behavior caused by mucin cross-linking. In addition, IL-13-activated HAECs shows increased peroxidase activity in apical secretions, and an overlaid thiolated po","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-05-02T12:45:08.133Z","creation":"2025-04-04T00:29:54.178Z"},"accession":"S-EPMC11383604","cross_references":{"pubmed":["38889046"],"doi":["10.1172/jci.insight.181024"]}}