biostudies-literatureLing XBeijing Medical Award FoundationHaiyan Foundation of Harbin Medical University Cancer HospitalNational Natural Science Foundation of China310https://www.ebi.ac.uk/biostudies/studies/S-EPMC11385129biostudies-literatureUnknown24(1)<h4>Background</h4>Phosphofructokinase P (PFKP) is a key rate-limiting enzyme in glycolysis, playing a crucial role in various pathophysiological processes. However, its specific function in tumors remains unclear. This study aims to evaluate the expression and specific role of PFKP across multiple tumor types (Pan-cancer) and to explore its potential clinical significance as a therapeutic target in cancer treatment.<h4>Methods</h4>We analyzed the expression of PFKP, immune cell infiltration, and patient prognosis across various cancers using data from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Additionally, we conducted a series of experiments in lung cancer cells, including Western blot, CCK-8 assay, colony formation assay, transwell migration assay, scraCancer cell internationalA comprehensive prognostic and immunological implications of PFKP in pan-cancer.PMC1138512982172786JJMS2023-04No. YXJL-2023-0091-0054Ling XLiang HFang CMa JZhang LfalseA comprehensive prognostic and immunological implications of PFKP in pan-cancer.<h4>Background</h4>Phosphofructokinase P (PFKP) is a key rate-limiting enzyme in glycolysis, playing a crucial role in various pathophysiological processes. However, its specific function in tumors remains unclear. This study aims to evaluate the expression and specific role of PFKP across multiple tumor types (Pan-cancer) and to explore its potential clinical significance as a therapeutic target in cancer treatment.<h4>Methods</h4>We analyzed the expression of PFKP, immune cell infiltration, and patient prognosis across various cancers using data from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Additionally, we conducted a series of experiments in lung cancer cells, including Western blot, CCK-8 assay, colony formation assay, transwell migration assay, scra2024-01-01T00:00:00Z2024 Sep2025-04-04T12:10:17.007Z2025-04-04T12:10:17.007ZS-EPMC113851293925201410.1186/s12935-024-03497-w