biostudies-literatureUnknown48(5)Yang Y<h4>Background</h4>With the prevalence of dietary supplements, the use of combinations of herbs and drugs is gradually increasing, together with the risk of drug interactions. In our clinical work, we unexpectedly found that the combination of Panax notoginseng and warfarin, which are herbs that activate blood circulation and remove blood stasis, showed antagonistic effects instead. The purpose of this study was to evaluate the drug interaction between Panax <i>notoginseng saponins</i> (PNS) and warfarin, the main active ingredient of Panax notoginseng, and to explore the interaction mechanism.<h4>Methods</h4>The effects and mechanisms of PNS on the pharmacodynamics and pharmacokinetics of warfarin were explored mainly in Sprague-Dawley rats and HepG2 cells. Elisa was used to detect the coJournal of ginseng research494-503https://www.ebi.ac.uk/biostudies/studies/S-EPMC11385396biostudies-literatureMechanism of Panax notoginseng saponins modulation of miR-214-3p/NR1I3 affecting the pharmacodynamics and pharmacokinetics of warfarin.PMC11385396Yang YXia ZLi JShao JZhai ZHe LYao HfalseMechanism of Panax notoginseng saponins modulation of miR-214-3p/NR1I3 affecting the pharmacodynamics and pharmacokinetics of warfarin.<h4>Background</h4>With the prevalence of dietary supplements, the use of combinations of herbs and drugs is gradually increasing, together with the risk of drug interactions. In our clinical work, we unexpectedly found that the combination of Panax notoginseng and warfarin, which are herbs that activate blood circulation and remove blood stasis, showed antagonistic effects instead. The purpose of this study was to evaluate the drug interaction between Panax <i>notoginseng saponins</i> (PNS) and warfarin, the main active ingredient of Panax notoginseng, and to explore the interaction mechanism.<h4>Methods</h4>The effects and mechanisms of PNS on the pharmacodynamics and pharmacokinetics of warfarin were explored mainly in Sprague-Dawley rats and HepG2 cells. Elisa was used to detect the co2024-01-01T00:00:00Z2024 Sep2025-04-18T13:38:33.248Z2025-04-04T11:34:01.303ZS-EPMC113853963926330710.1016/j.jgr.2024.05.003