biostudies-literatureUnknown14(1)Diakos EFish domestication progresses through five levels: from the initial acclimatization to captivity (Level 1), to the life cycle completion in captivity (Level 4), and even to the implementation of selective breeding programs (Level 5). Domestication leads to phenotypic changes over generations, sometimes from the very first generation. Behavioral traits are among the first to change. However, in fish, potential behavioral changes during early domestication have been little studied. Therefore, we studied potential behavioral changes among early and advanced levels of domestication in a model species, the zebrafish (Danio rerio), using a mirror test experiment, commonly used to assess traits involved in activity, aggressiveness, and stress in this species. We compared these traits between wildScientific reports21036https://www.ebi.ac.uk/biostudies/studies/S-EPMC11385545biostudies-literatureEarly impact of domestication on aggressiveness, activity, and stress behaviors in zebrafish (Danio rerio) using mirror test and automated videotracking.PMC11385545Chevalier CHardy APasquet ADiakos EKestemont PFontaine PShahjahan MLambert SLecocq TfalseEarly impact of domestication on aggressiveness, activity, and stress behaviors in zebrafish (Danio rerio) using mirror test and automated videotracking.Fish domestication progresses through five levels: from the initial acclimatization to captivity (Level 1), to the life cycle completion in captivity (Level 4), and even to the implementation of selective breeding programs (Level 5). Domestication leads to phenotypic changes over generations, sometimes from the very first generation. Behavioral traits are among the first to change. However, in fish, potential behavioral changes during early domestication have been little studied. Therefore, we studied potential behavioral changes among early and advanced levels of domestication in a model species, the zebrafish (Danio rerio), using a mirror test experiment, commonly used to assess traits involved in activity, aggressiveness, and stress in this species. We compared these traits between wild2024-01-01T00:00:00Z2024 Sep2026-04-08T16:15:00.621Z2025-04-04T02:50:46.984ZS-EPMC113855453925176610.1038/s41598-024-71451-x