biostudies-literatureUnknownMoreira RNatural Sciences and Engineering Research Council of CanadaNational Institute of Allergy and Infectious DiseasesNCRR NIH HHSNIAID NIH HHSNational Institutes of HealthNIGMS NIH HHSHere we describe bibacillin 1 - a two-component lantibiotic from <i>Bacillus thuringiensis</i>. The peptides that comprise bibacillin 1 are modified by a class II lanthipeptide synthetase Bib1M producing two peptides with non-overlapping ring patterns that are reminiscent of cerecidin and the short component of the enterococcal cytolysin (CylL_S''), a virulence factor associated with human disease. Stereochemical analysis demonstrated that each component contains ll-methyllanthionine and dl-lanthionine. The mature bibacillin 1 peptides showed cooperative bactericidal activity against Gram-positive bacteria, including members of the ESKAPE pathogens, and weak hemolytic activity. Optimal ratio studies suggest that bibacillin 1 works best when the components are present in a 1 : 1 rRSC chemical biologyhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC11385697biostudies-literatureBibacillin 1: a two-component lantibiotic from <i>Bacillus thuringiensis</i>.PMC11385697S10 RR027109 AR37GM058822S10 RR027109R37 GM058822AI083214P01 AI083214Moreira RYang YGilmore MSLuo Yvan der Donk WAfalseBibacillin 1: a two-component lantibiotic from <i>Bacillus thuringiensis</i>.Here we describe bibacillin 1 - a two-component lantibiotic from <i>Bacillus thuringiensis</i>. The peptides that comprise bibacillin 1 are modified by a class II lanthipeptide synthetase Bib1M producing two peptides with non-overlapping ring patterns that are reminiscent of cerecidin and the short component of the enterococcal cytolysin (CylL_S''), a virulence factor associated with human disease. Stereochemical analysis demonstrated that each component contains ll-methyllanthionine and dl-lanthionine. The mature bibacillin 1 peptides showed cooperative bactericidal activity against Gram-positive bacteria, including members of the ESKAPE pathogens, and weak hemolytic activity. Optimal ratio studies suggest that bibacillin 1 works best when the components are present in a 1 : 1 r2024-01-01T00:00:00Z2024 Sep2026-06-04T09:42:11.085Z2025-04-21T14:55:29.141ZS-EPMC113856973926854410.1039/d4cb00192c