{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(2)"],"submitter":["Li J"],"pubmed_abstract":["Biopharmaceutical glycoproteins produced in plants carry N-glycans with plant-specific residues core α(1,3)-fucose and β(1,2)-xylose, which can significantly impact the activity, stability and immunogenicity of biopharmaceuticals. In this study, we have employed sequence-specific transcription activator-like effector nucleases (TALENs) to knock out two α(1,3)-fucosyltransferase (FucT) and the two β(1,2)-xylosyltransferase (XylT) genes within Nicotiana benthamiana to generate plants with improved capacity to produce glycoproteins devoid of plant-specific residues. Among plants regenerated from N. benthamiana protoplasts transformed with TALENs targeting either the FucT or XylT genes, 50% (80 of 160) and 73% (94 of 129) had mutations in at least one FucT or XylT allele, respectively. Among p"],"journal":["Plant biotechnology journal"],"pagination":["533-42"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11389102"],"repository":["biostudies-literature"],"pubmed_title":["Multiplexed, targeted gene editing in Nicotiana benthamiana for glyco-engineering and monoclonal antibody production."],"pmcid":["PMC11389102"],"pubmed_authors":["Coffman AP","Zhang F","Li J","Clasen BM","Stoddard TJ","Mathis L","Yabandith A","Ray EE","D'Aoust MA","Demorest ZL","Voytas DF","Retterath AJ","Lavoie PO","Cedrone F","Luo S","Daulhac A"],"additional_accession":[]},"is_claimable":false,"name":"Multiplexed, targeted gene editing in Nicotiana benthamiana for glyco-engineering and monoclonal antibody production.","description":"Biopharmaceutical glycoproteins produced in plants carry N-glycans with plant-specific residues core α(1,3)-fucose and β(1,2)-xylose, which can significantly impact the activity, stability and immunogenicity of biopharmaceuticals. In this study, we have employed sequence-specific transcription activator-like effector nucleases (TALENs) to knock out two α(1,3)-fucosyltransferase (FucT) and the two β(1,2)-xylosyltransferase (XylT) genes within Nicotiana benthamiana to generate plants with improved capacity to produce glycoproteins devoid of plant-specific residues. Among plants regenerated from N. benthamiana protoplasts transformed with TALENs targeting either the FucT or XylT genes, 50% (80 of 160) and 73% (94 of 129) had mutations in at least one FucT or XylT allele, respectively. Among p","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Feb","modification":"2026-07-15T08:03:21.068Z","creation":"2025-04-19T14:36:07.311Z"},"accession":"S-EPMC11389102","cross_references":{"pubmed":["26011187"],"doi":["10.1111/pbi.12403"]}}