biostudies-literatureUnknown15Kim C<h4>Introduction</h4>Bruton's tyrosine kinase (BTK) and interleukin (IL)-2 Inducible T-cell Kinase (ITK) inhibitors have anti-inflammatory properties. We investigated the therapeutic effect of ibrutinib, an orally bioavailable BTK/ITK inhibitor, in a mouse model of Graves' orbitopathy (GO).<h4>Methods</h4>Genetic immunization was performed through intramuscular administration of the recombinant plasmid, pCMV6-hTSHR cDNA, to 8-week-old female BALB/c mice. Serum levels of T3, T4, and thyroid-stimulating hormone receptor (TSHR) antibodies (TRAbs) were quantified using enzyme-linked immunosorbent assay. Histopathological changes in orbital tissues were examined using immunohistochemistry (IHC) staining for TSHR and various inflammatory markers. Following successful genetic immunization, ibrutiFrontiers in endocrinology1420024https://www.ebi.ac.uk/biostudies/studies/S-EPMC11392736biostudies-literatureImpact of ibrutinib on inflammation in a mouse model of Graves' orbitopathy.PMC11392736Kim CYang JWChung JKPark JHJang SYChoi YJYoon JSJun HOPark TKfalseImpact of ibrutinib on inflammation in a mouse model of Graves' orbitopathy.<h4>Introduction</h4>Bruton's tyrosine kinase (BTK) and interleukin (IL)-2 Inducible T-cell Kinase (ITK) inhibitors have anti-inflammatory properties. We investigated the therapeutic effect of ibrutinib, an orally bioavailable BTK/ITK inhibitor, in a mouse model of Graves' orbitopathy (GO).<h4>Methods</h4>Genetic immunization was performed through intramuscular administration of the recombinant plasmid, pCMV6-hTSHR cDNA, to 8-week-old female BALB/c mice. Serum levels of T3, T4, and thyroid-stimulating hormone receptor (TSHR) antibodies (TRAbs) were quantified using enzyme-linked immunosorbent assay. Histopathological changes in orbital tissues were examined using immunohistochemistry (IHC) staining for TSHR and various inflammatory markers. Following successful genetic immunization, ibruti2024-01-01T00:00:00Z20242026-06-04T03:08:02.581Z2025-04-04T09:48:48.51ZS-EPMC113927363928000710.3389/fendo.2024.1420024