{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Ye J"],"funding":["NICHD NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["The miR-290 and miR-302 clusters of microRNAs are highly expressed in naïve and primed pluripotent stem cells, respectively. Ectopic expression of the embryonic stem cell-specific cell cycle regulating (ESCC) family of microRNAs arising from these two clusters dramatically enhances the reprogramming of both mouse and human somatic cells to induced pluripotency. Here, we used genetic knockouts to dissect the requirement for the miR-290 and miR-302 clusters during the reprogramming of mouse fibroblasts into induced pluripotent stem cells (iPSCs) with retrovirally introduced Oct4, Sox2, and Klf4. Knockout of either cluster alone did not negatively impact the efficiency of reprogramming. Resulting cells appeared identical to their embryonic stem cell microRNA cluster knockout counterparts. In "],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.09.02.610895"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11398367"],"repository":["biostudies-literature"],"pubmed_title":["The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells."],"pmcid":["PMC11398367"],"funding_grant_id":["T32 HD007263","F30 HD084120","F32 HD070572","R01 GM101180","T32 HD007470"],"pubmed_authors":["Blelloch R","Parchem RJ","Judson-Torres RL","Ye J","Boileau RM"],"additional_accession":[]},"is_claimable":false,"name":"The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.","description":"The miR-290 and miR-302 clusters of microRNAs are highly expressed in naïve and primed pluripotent stem cells, respectively. Ectopic expression of the embryonic stem cell-specific cell cycle regulating (ESCC) family of microRNAs arising from these two clusters dramatically enhances the reprogramming of both mouse and human somatic cells to induced pluripotency. Here, we used genetic knockouts to dissect the requirement for the miR-290 and miR-302 clusters during the reprogramming of mouse fibroblasts into induced pluripotent stem cells (iPSCs) with retrovirally introduced Oct4, Sox2, and Klf4. Knockout of either cluster alone did not negatively impact the efficiency of reprogramming. Resulting cells appeared identical to their embryonic stem cell microRNA cluster knockout counterparts. In ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2025-04-22T16:56:57.889Z","creation":"2025-04-06T01:55:38.061Z"},"accession":"S-EPMC11398367","cross_references":{"pubmed":["39282363"],"doi":["10.1101/2024.09.02.610895"]}}