{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(3)"],"submitter":["Hou Z"],"pubmed_abstract":["MicroRNAs (miRNAs) and short RNA fragments (18-25 nt) are crucial biomarkers in biological research and disease diagnostics. However, their accurate and rapid detection remains a challenge, largely due to their low abundance, short length, and sequence similarities. In this study, we report on a highly sensitive, one-step RNA O-circle amplification (ROA) assay for rapid and accurate miRNA detection. The ROA assay commences with the hybridization of a circular probe with the test RNA, followed by a linear rolling circle amplification (RCA) using dUTP. This amplification process is facilitated by U-nick reactions, which lead to an exponential amplification for readout. Under optimized conditions, assays can be completed within an hour, producing an amplification yield up to the microgram lev"],"journal":["aBIOTECH"],"pagination":["298-308"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11399362"],"repository":["biostudies-literature"],"pubmed_title":["A sensitive one-pot ROA assay for rapid miRNA detection."],"pmcid":["PMC11399362"],"pubmed_authors":["Ruan J","Hou Z","Guan X","Zhang Y","Li A","Wang X","Chang J","Deng W","Chang Y","Wang K"],"additional_accession":[]},"is_claimable":false,"name":"A sensitive one-pot ROA assay for rapid miRNA detection.","description":"MicroRNAs (miRNAs) and short RNA fragments (18-25 nt) are crucial biomarkers in biological research and disease diagnostics. However, their accurate and rapid detection remains a challenge, largely due to their low abundance, short length, and sequence similarities. In this study, we report on a highly sensitive, one-step RNA O-circle amplification (ROA) assay for rapid and accurate miRNA detection. The ROA assay commences with the hybridization of a circular probe with the test RNA, followed by a linear rolling circle amplification (RCA) using dUTP. This amplification process is facilitated by U-nick reactions, which lead to an exponential amplification for readout. Under optimized conditions, assays can be completed within an hour, producing an amplification yield up to the microgram lev","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2025-04-20T00:45:42.485Z","creation":"2025-04-20T00:45:42.485Z"},"accession":"S-EPMC11399362","cross_references":{"pubmed":["39279850"],"doi":["10.1007/s42994-024-00140-0"]}}