{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["64(3)"],"submitter":["Phian S"],"pubmed_abstract":["Bioinoculants of <i>Sphingobium indicum</i> B90A have been used to decontaminate hexachlorocyclohexane (HCH)-contaminated soils in the past. There is no selective or convenient method available to track the added B90A in HCH-contaminated soils in the presence of several native sphingomonads. Here, we describe a method, BioMarkTrack, for tracking B90A bioinoculant by simple amplification of the B90A specific biomarker genes. Whole-genome sequence data of 120 different genera of sphingomonads (<i>Sphingobium</i>, <i>Novosphingobium</i>, <i>Sphingomonas</i>, <i>Sphingopyxis</i>, and <i>Sphingosinicella</i>) were retrieved from the NCBI database and annotated. Intra- and inter-genus similarity searches, including the genome of B90A as a reference was conducted. 122 unique gene sequences were i"],"journal":["Indian journal of microbiology"],"pagination":["1266-1277"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11399485"],"repository":["biostudies-literature"],"pubmed_title":["Comparative genomics reveal unique markers to monitor by routine PCR assay bioinoculant of &lt;i&gt;Sphingobium indicum&lt;/i&gt; B90A in hexachlorocyclohexane (HCH) contaminated soils."],"pmcid":["PMC11399485"],"pubmed_authors":["Singh DN","Singh Y","Verma H","Lal R","Phian S","Rawat CD"],"additional_accession":[]},"is_claimable":false,"name":"Comparative genomics reveal unique markers to monitor by routine PCR assay bioinoculant of &lt;i&gt;Sphingobium indicum&lt;/i&gt; B90A in hexachlorocyclohexane (HCH) contaminated soils.","description":"Bioinoculants of <i>Sphingobium indicum</i> B90A have been used to decontaminate hexachlorocyclohexane (HCH)-contaminated soils in the past. There is no selective or convenient method available to track the added B90A in HCH-contaminated soils in the presence of several native sphingomonads. Here, we describe a method, BioMarkTrack, for tracking B90A bioinoculant by simple amplification of the B90A specific biomarker genes. Whole-genome sequence data of 120 different genera of sphingomonads (<i>Sphingobium</i>, <i>Novosphingobium</i>, <i>Sphingomonas</i>, <i>Sphingopyxis</i>, and <i>Sphingosinicella</i>) were retrieved from the NCBI database and annotated. Intra- and inter-genus similarity searches, including the genome of B90A as a reference was conducted. 122 unique gene sequences were i","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-05-29T06:56:52.639Z","creation":"2026-04-08T03:50:37.126Z"},"accession":"S-EPMC11399485","cross_references":{"pubmed":["39282163"],"doi":["10.1007/s12088-024-01321-7"]}}