biostudies-literatureUnknown64(3)Phian SBioinoculants of <i>Sphingobium indicum</i> B90A have been used to decontaminate hexachlorocyclohexane (HCH)-contaminated soils in the past. There is no selective or convenient method available to track the added B90A in HCH-contaminated soils in the presence of several native sphingomonads. Here, we describe a method, BioMarkTrack, for tracking B90A bioinoculant by simple amplification of the B90A specific biomarker genes. Whole-genome sequence data of 120 different genera of sphingomonads (<i>Sphingobium</i>, <i>Novosphingobium</i>, <i>Sphingomonas</i>, <i>Sphingopyxis</i>, and <i>Sphingosinicella</i>) were retrieved from the NCBI database and annotated. Intra- and inter-genus similarity searches, including the genome of B90A as a reference was conducted. 122 unique gene sequences were iIndian journal of microbiology1266-1277https://www.ebi.ac.uk/biostudies/studies/S-EPMC11399485biostudies-literatureComparative genomics reveal unique markers to monitor by routine PCR assay bioinoculant of <i>Sphingobium indicum</i> B90A in hexachlorocyclohexane (HCH) contaminated soils.PMC11399485Singh DNSingh YVerma HLal RPhian SRawat CDfalseComparative genomics reveal unique markers to monitor by routine PCR assay bioinoculant of <i>Sphingobium indicum</i> B90A in hexachlorocyclohexane (HCH) contaminated soils.Bioinoculants of <i>Sphingobium indicum</i> B90A have been used to decontaminate hexachlorocyclohexane (HCH)-contaminated soils in the past. There is no selective or convenient method available to track the added B90A in HCH-contaminated soils in the presence of several native sphingomonads. Here, we describe a method, BioMarkTrack, for tracking B90A bioinoculant by simple amplification of the B90A specific biomarker genes. Whole-genome sequence data of 120 different genera of sphingomonads (<i>Sphingobium</i>, <i>Novosphingobium</i>, <i>Sphingomonas</i>, <i>Sphingopyxis</i>, and <i>Sphingosinicella</i>) were retrieved from the NCBI database and annotated. Intra- and inter-genus similarity searches, including the genome of B90A as a reference was conducted. 122 unique gene sequences were i2024-01-01T00:00:00Z2024 Sep2026-05-29T06:56:52.639Z2026-04-08T03:50:37.126ZS-EPMC113994853928216310.1007/s12088-024-01321-7