biostudies-literatureVisvanathan KNCI NIH HHSThe John Fetting Fund for Breast Cancer Prevention9533461https://www.ebi.ac.uk/biostudies/studies/S-EPMC11401740biostudies-literatureUnknown2022<h4>Introduction</h4>Critical regulatory genes are functionally silenced by DNA hypermethylation in breast cancer and premalignant lesions. The objective of this study was to examine whether DNA methylation assessed in random fine needle aspirates (rFNA) can be used to inform breast cancer risk.<h4>Methods</h4>In 20 women with invasive breast cancer scheduled for surgery at Johns Hopkins Hospital, cumulative methylation status was assessed in a comprehensive manner. rFNA was performed on tumors, adjacent normal tissues, and all remaining quadrants. Pathology review was conducted on blocks from all excised tissue. The cumulative methylation index (CMI) for 12 genes was assessed by a highly sensitive QM-MSP assay in 280 aspirates and tissue from 11 incidental premalignant lesions. Mann-WhitnThe breast journalEvaluating DNA Methylation in Random Fine Needle Aspirates from the Breast to Inform Cancer Risk.PMC11401740P30 CA006973NCIT32CA009314T32 CA009314NCIP30CA006973Fetting JFackler MJCimino-Mathews ASukumar SMay BJacobs LOrellana MCamp MHabibi MVisvanathan KWhite MJStearns VKaria PSVandenBussche CJLange JEuhus DfalseEvaluating DNA Methylation in Random Fine Needle Aspirates from the Breast to Inform Cancer Risk.<h4>Introduction</h4>Critical regulatory genes are functionally silenced by DNA hypermethylation in breast cancer and premalignant lesions. The objective of this study was to examine whether DNA methylation assessed in random fine needle aspirates (rFNA) can be used to inform breast cancer risk.<h4>Methods</h4>In 20 women with invasive breast cancer scheduled for surgery at Johns Hopkins Hospital, cumulative methylation status was assessed in a comprehensive manner. rFNA was performed on tumors, adjacent normal tissues, and all remaining quadrants. Pathology review was conducted on blocks from all excised tissue. The cumulative methylation index (CMI) for 12 genes was assessed by a highly sensitive QM-MSP assay in 280 aspirates and tissue from 11 incidental premalignant lesions. Mann-Whitn2022-01-01T00:00:00Z20222026-06-02T03:17:14.833Z2026-06-02T03:11:33.914ZS-EPMC114017403974165410.1155/2022/9533461