{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Krishnan N"],"funding":["Rajiv Gandhi Centre for Biotechnology, Department of Biotechnology, Ministry of Science and Technology, India","University Grants Commission","Indian Council of Medical Research"],"pagination":["e37064"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11403530"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(17)"],"pubmed_abstract":["Objective
The role of β-hCG in breast cancer is largely unknown, this study aims to analyse the gene expression and clinical implications of β-hCG and its isoforms in various cancers focussing particularly in Breast Invasive Carcinoma (BRCA). A mechanistic approach deciphering the transcriptional regulation of β-hCG by BRCA1 was also explored.Methods
Data from various comprehensive gene expression platforms like UALCAN, GEPIA2, GENT2, TIMER2, LinkedOmics, and STRING were used to analyse the expression of β-hCG and its clinical implications; Immunohistochemistry and ELISA for β-hCG expression analysis from human breast cancer patients; Electrophoretic mobility shift assay (EMSA) to analyse the direct binding of BRCA1 on β-hCG; Immunoblotting and Luciferase assay to understan"],"journal":["Heliyon"],"pubmed_title":["Direct DNA binding by BRCA1 on β-hCG promoter and its clinical implications."],"pmcid":["PMC11403530"],"funding_grant_id":["EMDR/SGll3l 2023-2686"],"pubmed_authors":["Anandan J","Rajan A","V G M","S P","Mohammed S","Warrier AV","Sundaram S","Krishnan N","Nair RS","Ittycheria SS","Yadev I","Kumari P","Srinivas P","R L N"],"additional_accession":[]},"is_claimable":false,"name":"Direct DNA binding by BRCA1 on β-hCG promoter and its clinical implications.","description":"Objective
The role of β-hCG in breast cancer is largely unknown, this study aims to analyse the gene expression and clinical implications of β-hCG and its isoforms in various cancers focussing particularly in Breast Invasive Carcinoma (BRCA). A mechanistic approach deciphering the transcriptional regulation of β-hCG by BRCA1 was also explored.Methods
Data from various comprehensive gene expression platforms like UALCAN, GEPIA2, GENT2, TIMER2, LinkedOmics, and STRING were used to analyse the expression of β-hCG and its clinical implications; Immunohistochemistry and ELISA for β-hCG expression analysis from human breast cancer patients; Electrophoretic mobility shift assay (EMSA) to analyse the direct binding of BRCA1 on β-hCG; Immunoblotting and Luciferase assay to understan","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-06-03T16:56:39.438Z","creation":"2025-04-04T02:54:09.024Z"},"accession":"S-EPMC11403530","cross_references":{"pubmed":["39286065"],"doi":["10.1016/j.heliyon.2024.e37064"]}}